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. 1973 May;11(5):665–671. doi: 10.1128/jvi.11.5.665-671.1973

Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses. VI. Characterization of the DNA from Five Nondefective Hybrid Viruses

Patrick H Henry 1,2,3, Lowell E Schnipper 1,2,3, Richard J Samaha 1,2,3, Clyde S Crumpacker 1,2,3,1, Andrew M Lewis Jr 1,2,3, Arthur S Levine 1,2,3
PMCID: PMC355162  PMID: 4350711

Abstract

Certain biophysical characteristics of the DNA from each of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses (Ad2+ND1, Ad2+ND2, Ad2+ND3, Ad2+ND4, Ad2+ND5) have been determined. The guanine plus cytosine content varied from 55 to 57% and was not significantly different from that of nonhybrid Ad2 (56%), and the hybrid DNA molecules had mean molecular lengths which were similar to that of the standard, Ad2. The Ad2 and SV40 components of each hybrid were linked by alkali-resistant, presumably covalent bonds. The percentage of SV40 DNA in each hybrid virus was determined by hybridization with SV40 complementary RNA in a calibrated system. The results indicate that each hybrid virus DNA contains a different percentage of SV40 nucleotide sequences. The estimated size of the SV40 DNA component varies from 48,000 daltons for Ad2+ND3 to 840,000 daltons for Ad2+ND4, the latter being equivalent to between one-fourth and one-third of the SV40 genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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