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. 1973 May;11(5):682–693. doi: 10.1128/jvi.11.5.682-693.1973

Preferred Site for Initiation of RNA Transcription by Escherichia coli RNA Polymerase Within the Simian Virus 40 DNA Segment of the Nondefective Adenovirus-Simian Virus 40 Hybrid Viruses Ad2+ND1 and Ad2+ND3

B Sayeeda Zain 1,2, Ravi Dhar 1,2, Sherman M Weissman 1,2, Paul Lebowitz 1,2, Andrew M Lewis Jr 1,2
PMCID: PMC355164  PMID: 4350713

Abstract

The DNA of simian virus 40 (SV40) was transcribed into RNA by Escherichia coli RNA polymerase at 18 to 24 C after synchronization of the initiation of RNA synthesis. After a brief synthetic period the RNA product contained relatively large amounts of sequences derived from a limited segment of SV40 DNA. The source for this pulse-labeled RNA was found to be a portion of the segment of SV40 DNA included within the nondefective adenovirus (Ad)-SV40 hybrid viruses, Ad2+ND1 and Ad2+ND3. After synthesis with [γ-32P] ATP, Ad2+ND1 and Ad2+ND3 DNA transcripts contained an initial sequence missing from Ad2 transcripts. This sequence was identified as an initiation sequence for polymerase transcription of the SV40 DNA. Thus, there is a preferred site for initiation of in vitro transcription on the segment of SV40 DNA common to the nondefective Ad2+ND1 and Ad2+ND3 hybrid viruses.

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