Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1973 May;11(5):756–760. doi: 10.1128/jvi.11.5.756-760.1973

Genetic Study of Suppressor-Sensitive Mutants of the Bacillus subtilis Bacteriophage φ29

Bernard E Reilly 1, Viola M Zeece 1, Dwight L Anderson 1
PMCID: PMC355173  PMID: 4196635

Abstract

With bacteriophage φ29 of Bacillus subtilis 133, suppressor-sensitive (sus) hydroxylamine mutants have been isolated. Intracistronic and intercistronic quantitative complementation placed the mutants in 13 cistrons, and three-factor crosses have been used to assign an unambiguous order for 10 cistrons. Recombination frequencies have been presented for several regions of the genome to facilitate comparison of the sus system with the previously published temperature-sensitive mapping systems.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. L., Hickman D. D., Reilly B. E. Structure of Bacillus subtilis bacteriophage phi 29 and the length of phi 29 deoxyribonucleic acid. J Bacteriol. 1966 May;91(5):2081–2089. doi: 10.1128/jb.91.5.2081-2089.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson D. L., Mosharrafa E. T. Physical and biological properties of phage phi 29 deoxyribonucleic acid. J Virol. 1968 Oct;2(10):1185–1190. doi: 10.1128/jvi.2.10.1185-1190.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FREESE E., BAUTZ E., FREESE E. B. The chemical and mutagenic specificity of hydroxylamine. Proc Natl Acad Sci U S A. 1961 Jun 15;47:845–855. doi: 10.1073/pnas.47.6.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Georgopoulos C. P. Suppressor system in Bacillus subtilis 168. J Bacteriol. 1969 Mar;97(3):1397–1402. doi: 10.1128/jb.97.3.1397-1402.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hagen E. W., Zeece V. M., Anderson D. L. A genetic study of temperature-sensitive mutants of the Bacillus subtilis bacteriophage phi 29. Virology. 1971 Mar;43(3):561–568. doi: 10.1016/0042-6822(71)90281-9. [DOI] [PubMed] [Google Scholar]
  6. Mosharrafa E. T., Schachtele C. F., Reilly B. E., Anderson D. L. Complementary Strands of Bacteriophage phi29 Deoxyribonucleic Acid: Preparative Separation and Transcription Studies. J Virol. 1970 Dec;6(6):855–864. doi: 10.1128/jvi.6.6.855-864.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ortin J., Viñuela E., Salas M., Vasquez C. DNA-protein complex in circular DNA from phage phi-29. Nat New Biol. 1971 Dec 29;234(52):275–277. doi: 10.1038/newbio234275a0. [DOI] [PubMed] [Google Scholar]
  8. REILLY B. E., SPIZIZEN J. BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS. J Bacteriol. 1965 Mar;89:782–790. doi: 10.1128/jb.89.3.782-790.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. TESSMAN I., PODDAR R. K., KUMAR S. IDENTIFICATION OF THE ALTERED BASES IN MUTATED SINGLE-STRANDED DNA. I. IN VITRO MUTAGENESIS BY HYDROXYLAMINE, ETHYL METHANESULFONATE AND NITROUS ACID. J Mol Biol. 1964 Aug;9:352–363. doi: 10.1016/s0022-2836(64)80212-6. [DOI] [PubMed] [Google Scholar]
  10. Talavera A., Jimenez F., Salas M., Viñuela E. Mapping of temperature sensitive mutants of bacteriophage phi 29. Mol Gen Genet. 1972;115(1):31–35. doi: 10.1007/BF00272215. [DOI] [PubMed] [Google Scholar]
  11. Talavera A., Jimenez F., Salas M., Viñuela E. Temperature-sensitive mutants of bacteriophage phi-29. Virology. 1971 Dec;46(3):586–595. doi: 10.1016/0042-6822(71)90062-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES