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. 1973 Jun;11(6):900–914. doi: 10.1128/jvi.11.6.900-914.1973

Semliki Forest Virus-Specific RNAs Synthesized In Vitro by Enzyme from Infected BHK Cells

Marcel R Michel 1, Peter J Gomatos 1
PMCID: PMC355198  PMID: 4736536

Abstract

When Semliki Forest virus (SFV)-infected BHK cells were disrupted 4 h after infection, 75 to 90% of the total virus-specific RNA synthesizing enzyme was found in the large particle fraction, along with 75 to 90% of the in vivo-synthesized double-stranded RNAs. The RNA products of this enzyme-template complex in an in vitro system were double-stranded RNAs sedimenting predominantly at 18S, and single-stranded RNAs sedimenting at 42S, 26S, and 22S. The various virus-specific SFV RNAs synthesized in vitro were associated with different sized structures, and thus each was separable by differential centrifugation. Kinetic and pulse-chase experiments showed that the double-stranded RNAs were the precursors to the single-stranded RNAs. There were several double-stranded RNAs identified both in the in vitro product and also in extracts from infected cells. The major replicative form had a molecular weight of 4.4 × 106.

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Selected References

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