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. 1973 Jun;11(6):915–925. doi: 10.1128/jvi.11.6.915-925.1973

Complementary RNA Species Isolated from Vesicular Stomatitis (HR Strain) Defective Virions

Polly Roy 1, P Repik 1, E Hefti 1, D H L Bishop 1
PMCID: PMC355199  PMID: 4351460

Abstract

The wild-type strain of vesicular stomatitis virus (VSV) contains in its complete virion (VSV-1, B particles) a minus strand RNA. The principle defective particle of the wild-type strain (VSV-111, T particles) contains a shorter minus strand, homologous to part of the VSV-1 genome. Neither virion contains any detectable complementary (plus) strand RNA. In contrast, a preparation of a heat-resistant (HR) strain of VSV containing defective virions was found to contain both plus (21%) and minus strand RNA, present in several distinct size classes. It was found that the RNA in the HR virion preparation was at least 94% single-stranded and principally (96%) in ribonucleoprotein complexes. On extraction the plus and minus strand RNA species partially annealed to give a population of double- and multistranded RNA species. A small amount of RNA polymerase activity was associated with the HR defective virus preparation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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