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. 1973 Jun;11(6):926–932. doi: 10.1128/jvi.11.6.926-932.1973

Persistent Infection of Cells in Culture by Measles Virus III. Comparison of Virus-Specific RNA Synthesized in Primary and Persistent Infection in HeLa Cells

Stuart H Winston 1,2, Robert Rustigian 1,2, Michael A Bratt 1,2
PMCID: PMC355200  PMID: 4576519

Abstract

The pattern of actinomycin D-resistant RNA synthesis was examined during primary infection of HeLa cells by virulent Edmonston measles virus and in two HeLa clones persistently infected by the same strain of virus. One of these clones, K11, produces infectious virus of low virulence for HeLa cells, and the other, K11A-HG-1, has thus far failed to yield infectious virus. The patterns of virus-specific RNA synthesized in these three types of infection are qualitatively similar to each other and to the patterns of virus-specific RNA synthesis in other paramyxovirus infections. There were, however, quantitative differences. In addition, virions of the virulent Edmonston strain of measles virus were found to contain high-molecular-weight RNA with a sedimentation constant identical to that of Newcastle disease virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adels B. R., Gajdusek C., Gibbs C. J., Jr, Albrecht P., Rogers N. G. Attempts to transmit subacute sclerosing panencephalitis and isolate a measles related agent, with a study of the immune response in patients and experimental animals. Neurology. 1968 Jan;18(1 Pt 2):30–51. doi: 10.1212/wnl.18.1_part_2.030. [DOI] [PubMed] [Google Scholar]
  2. Blair C. D., Robinson W. S. Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology. 1968 Aug;35(4):537–549. doi: 10.1016/0042-6822(68)90284-5. [DOI] [PubMed] [Google Scholar]
  3. Bratt M. A., Gallaher W. R. Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proc Natl Acad Sci U S A. 1969 Oct;64(2):536–543. doi: 10.1073/pnas.64.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bratt M. A. RNA synthesis in chick embryo cells infected with different strains of NDV. Virology. 1969 Jul;38(3):485–488. doi: 10.1016/0042-6822(69)90163-9. [DOI] [PubMed] [Google Scholar]
  5. Bratt M. A., Robinson W. S. Evidence for an RNA replicative intermediate in cells infected with Newcastle disease virus. J Gen Virol. 1971 Feb;10(2):139–145. doi: 10.1099/0022-1317-10-2-139. [DOI] [PubMed] [Google Scholar]
  6. Clavell L. A., Bratt M. A. Relationship between the ribonucleic acid synthesizing capacity of ultraviolet-irradiated Newcastle disease virus and its ability to induce interferon. J Virol. 1971 Oct;8(4):500–508. doi: 10.1128/jvi.8.4.500-508.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Compans R. W., Choppin P. W. The nucleic acid of the parainfluenza virus SV5. Virology. 1968 Jun;35(2):289–296. doi: 10.1016/0042-6822(68)90269-9. [DOI] [PubMed] [Google Scholar]
  8. Duesberg P. H., Robinson W. S. Isolation of the nucleic acid of Newcastle disease virus (NDV). Proc Natl Acad Sci U S A. 1965 Sep;54(3):794–800. doi: 10.1073/pnas.54.3.794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. East J. L., Kingsbury D. W. Mumps virus replication in chick embryo lung cells: properties of ribonucleic acids in virions and infected cells. J Virol. 1971 Aug;8(2):161–173. doi: 10.1128/jvi.8.2.161-173.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Horta-Barbosa L., Fuccillo D. A., Sever J. L. Chronic viral infections of the central nervous system. JAMA. 1971 Nov 22;218(8):1185–1188. [PubMed] [Google Scholar]
  11. Johnson R. T. Subacute sclerosing panencephalitis. J Infect Dis. 1970 Feb;121(2):227–230. doi: 10.1093/infdis/121.2.227. [DOI] [PubMed] [Google Scholar]
  12. KOHN A., YASSKY D. Growth of measles virus in KB cells. Virology. 1962 May;17:157–163. doi: 10.1016/0042-6822(62)90092-2. [DOI] [PubMed] [Google Scholar]
  13. Kingsbury D. W. Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. J Mol Biol. 1966 Jun;18(1):204–214. doi: 10.1016/s0022-2836(66)80086-4. [DOI] [PubMed] [Google Scholar]
  14. Kingsbury D. W., Portner A. On the genesis of incomplete Sendai virions. Virology. 1970 Dec;42(4):872–879. doi: 10.1016/0042-6822(70)90336-3. [DOI] [PubMed] [Google Scholar]
  15. Matumoto M. Multiplication of measles virus in cell cultures. Bacteriol Rev. 1966 Mar;30(1):152–176. doi: 10.1128/br.30.1.152-176.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mirchamsy H., Rapp F. Role of interferon in replication of virulent and attenuated strains of measles virus. J Gen Virol. 1969 Jun;4(4):513–522. doi: 10.1099/0022-1317-4-4-513. [DOI] [PubMed] [Google Scholar]
  17. PUCK T. T., MARCUS P. I., CIECIURA S. J. Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer. J Exp Med. 1956 Feb 1;103(2):273–283. doi: 10.1084/jem.103.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. RUSTIGIAN R. A carrier state in HeLa cells with measles virus (Edmonston strain) apparently associated with non-infectious virus. Virology. 1962 Jan;16:101–104. doi: 10.1016/0042-6822(62)90212-x. [DOI] [PubMed] [Google Scholar]
  19. Rustigian R. Persistent infection of cells in culture by measles virus. I. Development and characteristics of HeLa sublines persistently infected with complete virus. J Bacteriol. 1966 Dec;92(6):1792–1804. doi: 10.1128/jb.92.6.1792-1804.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rustigian R. Persistent infection of cells in culture by measles virus. II. Effect of measles antibody on persistently infected HeLa sublines and recovery of a HeLa clonal line persistently infected with incomplete virus. J Bacteriol. 1966 Dec;92(6):1805–1811. doi: 10.1128/jb.92.6.1805-1811.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schluederberg A. Measles virus RNA. Biochem Biophys Res Commun. 1971 Mar 19;42(6):1012–1015. doi: 10.1016/0006-291x(71)90004-0. [DOI] [PubMed] [Google Scholar]
  22. Shishido A., Yamanouchi K., Hikita M., Sato T., Fukuda A., Kobune F. Development of a cell culture system susceptible to measle, canine distemper, and rinderpest viruses. Arch Gesamte Virusforsch. 1967;22(3):364–380. doi: 10.1007/BF01242957. [DOI] [PubMed] [Google Scholar]
  23. UNDERWOOD G. E. Studies on measles virus in tissue culture. I. Growth rates in various cells and development of a plaque assay. J Immunol. 1959 Aug;83:198–205. [PubMed] [Google Scholar]

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