Abstract
When UV-irradiated T4 bacteriophage (v+) infects in the presence of chloramphenicol, the phage DNA rapidly acquires single-stranded breaks proportional to the dose of UV. In contrast, when UV-irradiated T4 v1 (radiation sensitive mutant) infects under identical conditions, the phage DNA remains integral. A series of coinfections with v+ and v1 phage (UV-v1 + majority non-UV-v+ and UV-v+ and majority non-UV-v1) show that the enzyme responsible for breakage is injected by the phage. It is also demonstrated that the v1 phage injects an inactive enzyme that delays breakage by the v+ enzyme and interferes with subsequent repair. The cross of v+ and v1 phage produces mixed progeny that contain both active and inactive enzyme in a single capsid. The possible function of this breaking enzyme, necessitating injection of multiple copies, is considered.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HARM W. Mutants of phage T4 with increased sensitivity to ultraviolet. Virology. 1963 Jan;19:66–71. doi: 10.1016/0042-6822(63)90025-4. [DOI] [PubMed] [Google Scholar]
- Kozinski A. W., Kozinski P. B., James R. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. I. Tertiary structure of early replicative and recombining deoxyribonucleic acid. J Virol. 1967 Aug;1(4):758–770. doi: 10.1128/jvi.1.4.758-770.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kozinski A., Lorkiewicz Z. K. Early intracellular events in the replication of T4 phage DNA, IV. Host-mediated single-stranded breaks and repair in ultraviolet-damaged T4 DNA. Proc Natl Acad Sci U S A. 1967 Nov;58(5):2109–2116. doi: 10.1073/pnas.58.5.2109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Litwin S., Shahn E., Kozinski A. W. Interpretation of sucrose gradient sedimentation pattern of deoxyribonucleic acid fragments resulting from random breaks. J Virol. 1969 Jul;4(1):24–30. doi: 10.1128/jvi.4.1.24-30.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller R. C., Jr, Kozinski A. W. Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. V. Further studies on the T4 protein-deoxyribonucleic acid complex. J Virol. 1970 Apr;5(4):490–501. doi: 10.1128/jvi.5.4.490-501.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller R. C., Kozinski A. W., Litwin S. Molecular Recombination in T4 Bacteriophage Deoxyribonucleic Acid: III. Formation of Long Single Strands During Recombination. J Virol. 1970 Mar;5(3):368–380. doi: 10.1128/jvi.5.3.368-380.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Showe M. K., Black L. W. Assembly core of bacteriophage T4: an intermediate in head formation. Nat New Biol. 1973 Mar 21;242(116):70–75. doi: 10.1038/newbio242070a0. [DOI] [PubMed] [Google Scholar]
- Yasuda S., Sekiguchi M. Mechanism of repair of DNA in bacteriophage. II. Inability of ultraviolet-sensitive strains of bacteriophage in inducing an enzyme activity to excise pyrimidine dimers. J Mol Biol. 1970 Jan 28;47(2):243–255. doi: 10.1016/0022-2836(70)90343-8. [DOI] [PubMed] [Google Scholar]