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. 1973 Jul;12(1):49–57. doi: 10.1128/jvi.12.1.49-57.1973

Influence of Gene 55 on the Regulation of Synthesis of Some Early Enzymes in Bacteriophage T4-Infected Escherichia coli

Christina Bolund 1
PMCID: PMC355228  PMID: 4353500

Abstract

In Escherichia coli B infected with phage T4 with mutations in gene 55 the shut-off of synthesis of two early enzymes was found to be delayed 10 to 15 min as compared to wild-type T4 infection. The kinetics of early enzyme formation was very similar to that obtained after DNA-negative mutant infection. The transcription of gene 1 (one of the genes coded for early enzymes) ceased at 9 to 12 min after infection whether the gene 55 product was active or not, as determined by the time dependence of fluorouracil rescuability of DNA synthesis in cells infected with a gene 1 amber mutant. The synthesis of the major part of the early RNA species, determined by pulse-labeling with uracil-5-3H, was furthermore found to be regulated independently of gene 55. An explanation for the extended early enzyme synthesis in DNA-negative and gene 55 mutant infection is offered: translation of early messengers is restricted after about 12 min in wild-type infection, whereas in cells infected with DNA-negative or gene 55 mutants, i.e., the absence of late protein synthesis, early messengers direct the formation of early enzymes for a longer time.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolle A., Epstein R. H., Salser W., Geiduschek E. P. Transcription during bacteriophage T4 development: requirements for late messenger synthesis. J Mol Biol. 1968 Apr 28;33(2):339–362. doi: 10.1016/0022-2836(68)90193-9. [DOI] [PubMed] [Google Scholar]
  2. Bolund C., Sköld O. Formation of RNA in T4 phage-infected bacteria: transcriptional regulation of early enzyme synthesis. Virology. 1971 Feb;43(2):390–402. doi: 10.1016/0042-6822(71)90311-4. [DOI] [PubMed] [Google Scholar]
  3. Bolund C., Sköld O. Regulation of early mRNA synthesis in bacteriophage T4-infected bacteria: dependence on bacteriophage-specific protein synthesis. J Virol. 1973 Jul;12(1):39–48. doi: 10.1128/jvi.12.1.39-48.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen S. S., Flaks J. G., Barner H. D., Loeb M. R., Lichtenstein J. THE MODE OF ACTION OF 5-FLUOROURACIL AND ITS DERIVATIVES. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1004–1012. doi: 10.1073/pnas.44.10.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRASER D., JERREL E. A. The amino acid composition of T3 bacteriophage. J Biol Chem. 1953 Nov;205(1):291–295. [PubMed] [Google Scholar]
  6. Greene R., Korn D. The instability of T4 messenger RNA. J Mol Biol. 1967 Sep 28;28(3):435–443. doi: 10.1016/s0022-2836(67)80092-5. [DOI] [PubMed] [Google Scholar]
  7. Guha A., Szybalski W., Salser W., Geiduschek E. P., Pulitzer J. F., Bolle A. Controls and polarity of transcription during bacteriophage T4 development. J Mol Biol. 1971 Jul 28;59(2):329–349. doi: 10.1016/0022-2836(71)90054-4. [DOI] [PubMed] [Google Scholar]
  8. Hosoda J., Levinthal C. Protein synthesis by Escherichia coli infected with bacteriophage T4D. Virology. 1968 Apr;34(4):709–727. doi: 10.1016/0042-6822(68)90092-5. [DOI] [PubMed] [Google Scholar]
  9. Ihler G., Nakada D. Role of initiation factors in the control of T4-specific protein synthesis. J Mol Biol. 1971 Dec 14;62(2):419–421. doi: 10.1016/0022-2836(71)90439-6. [DOI] [PubMed] [Google Scholar]
  10. Mathews C. K., Kessin R. H. Control of bacteriophage-induced enzyme synthesis in cells infected with a temperature-sensitive mutant. J Virol. 1967 Feb;1(1):92–96. doi: 10.1128/jvi.1.1.92-96.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pollack Y., Groner Y., Aviv(Greenshpan) H., Revel M. Role of initiation factor B (F3) in the preferential translation of T4 late messenger RNA in T4 infected E. Coli. FEBS Lett. 1970 Aug 17;9(4):218–221. doi: 10.1016/0014-5793(70)80359-3. [DOI] [PubMed] [Google Scholar]
  12. Pulitzer J. F. Function of T4 gene 55. I. Characterization of temperature-sensitive mutations in the "maturation" gene 55. J Mol Biol. 1970 Apr 28;49(2):473–488. doi: 10.1016/0022-2836(70)90258-5. [DOI] [PubMed] [Google Scholar]
  13. Pulitzer J. F., Geiduschek E. P. Function of T4 gene 55. II. RNA synthesis by temperature-sensitive gene 55 mutants. J Mol Biol. 1970 Apr 28;49(2):489–507. doi: 10.1016/0022-2836(70)90259-7. [DOI] [PubMed] [Google Scholar]
  14. Rosen B., Rothman F., Weigert M. G. Miscoding caused by 5-fluorouracil. J Mol Biol. 1969 Sep 14;44(2):363–375. doi: 10.1016/0022-2836(69)90181-8. [DOI] [PubMed] [Google Scholar]
  15. Salser W., Bolle A., Epstein R. Transcription during bacteriophage T4 development: a demonstration that distinct subclasses of the "early" RNA appear at different times and that some are "turned off" at late times. J Mol Biol. 1970 Apr 28;49(2):271–295. doi: 10.1016/0022-2836(70)90246-9. [DOI] [PubMed] [Google Scholar]
  16. Sercarz E. The production of phage structural proteins in single cells of Escherichia coli infected with phage T4. Virology. 1966 Feb;28(2):339–345. doi: 10.1016/0042-6822(66)90158-9. [DOI] [PubMed] [Google Scholar]
  17. Sköld O. Regulation of early RNA synthesis in bacteriophage T4-infected Escherichia coli cells. J Mol Biol. 1970 Nov 14;53(3):339–356. doi: 10.1016/0022-2836(70)90070-7. [DOI] [PubMed] [Google Scholar]
  18. Stevens A. New small polypeptides associated with DNA-dependent RNA polymerase of Escherichia coli after infection with bacteriophage T4. Proc Natl Acad Sci U S A. 1972 Mar;69(3):603–607. doi: 10.1073/pnas.69.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WIBERG J. S., BUCHANAN J. M. STUDIES ON LABILE DEOXYCYTIDYLATE HYDROXYMETHYLASES FROM ESCHERICHIA COLI B INFECTED WITH TEMPERATURE-SENSITIVE MUTANTS OF BACTERIOPHAGE T4. Proc Natl Acad Sci U S A. 1964 Mar;51:421–428. doi: 10.1073/pnas.51.3.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WIBERG J. S., DIRKSEN M. L., EPSTEIN R. H., LURIA S. E., BUCHANAN J. M. Early enzyme synthesis and its control in E. coli infected with some amber mutants of bacteriophage T4. Proc Natl Acad Sci U S A. 1962 Feb;48:293–302. doi: 10.1073/pnas.48.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilhelm J. M., Haselkorn R. In vitro synthesis of T4 proteins: the products of genes 9, 18, 19, 23, 24, and 38. Virology. 1971 Jan;43(1):198–208. doi: 10.1016/0042-6822(71)90237-6. [DOI] [PubMed] [Google Scholar]
  22. Young E. T., 2nd, van Houwe G. Control of synthesis of glucosyl transferase and lysozyme messengers after T4 infection. J Mol Biol. 1970 Aug;51(3):605–619. doi: 10.1016/0022-2836(70)90011-2. [DOI] [PubMed] [Google Scholar]

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