Fig. 4.
Comparison of the efficiency of different pseudovirions in infection of murine skin and cervicovaginal tissues. Mice were concomitantly challenged on the skin of the back and in the cervicovaginal tract with pseudovirions carrying an encapsidated luciferase-expressing pseudogenome. The amount for each inoculation corresponded to 1×109 pseudogenomes, as determined by qPCR. Mice that had been mock-infected with phosphate-buffered saline served as controls. Readout was performed after intraperitoneal administration of the luciferin substrate by in vivo imaging analysis on days two and three post infection. Each experimental group consisted of five animals and results of the average radiance are given in mean p/s/cm2/sr ± standard deviation.