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. 1974 Jan;13(1):1–8. doi: 10.1128/jvi.13.1.1-8.1974

Biochemical Studies on the χ Mutation of Bacteriophage T4: Differential Inhibition of χ+ and χ DNA Synthesis by Mitomycin C

Kenji Shimizu 1, Mutsuo Sekiguchi 1
PMCID: PMC355251  PMID: 4359423

Abstract

Biochemical studies were carried out to determine the effect of χ mutation on T4 DNA synthesis. The rate and final extent of DNA synthesis are almost the same with T4D- and T4χ-infected cells, although the burst size of T4χ is about one-sixth that of the wild type. The DNA synthesis of T4χ-infected cells is more readily inhibited by mitomycin C than is that of T4 wild type. When mitomycin C was added during active phage growth, DNA synthesis of T4χ halted almost immediately. T4 DNA polymerases isolated from χ+- and χ-infected cells, however, exhibit no difference with regard to their sensitivities to mitomycin C, priming activities with alkylated or ultraviolet light-irradiated templates and other enzymatic properties.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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