Abstract
Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mild detergent treatment and subsequent recovery of immune complexes showed this technique to be useful for the isolation of viral envelope antigens.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Atwell J. L., Cone R. E., Marchalonis J. J. Isolation of theta antigen from the surface of thymus lymphocytes. Nat New Biol. 1973 Feb 21;241(112):251–252. doi: 10.1038/newbio241251a0. [DOI] [PubMed] [Google Scholar]
- Day E. D., Lassiter S., Fritz R. B. Radioiodination of antibodies and adsorbed to insoluble antigens. J Immunol. 1967 Jan;98(1):67–71. [PubMed] [Google Scholar]
- Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses. J Virol. 1971 Nov;8(5):778–785. doi: 10.1128/jvi.8.5.778-785.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fritz R. B., Langlois A. J., Beard J. W. Reaction of chick embryo cells infected with leukosis strain MC29 virus with fluorescein-labeled antibody. J Virol. 1969 Oct;4(4):372–379. doi: 10.1128/jvi.4.4.372-379.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fritz R. B., Qualtiere L. F. Avian oncornavirus group-specific antigen: detection and quantification by radioimmunoassay. J Virol. 1973 May;11(5):736–740. doi: 10.1128/jvi.11.5.736-740.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hubbard A. L., Cohn Z. A. The enzymatic iodination of the red cell membrane. J Cell Biol. 1972 Nov;55(2):390–405. doi: 10.1083/jcb.55.2.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marchalonis J. J. An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem J. 1969 Jun;113(2):299–305. doi: 10.1042/bj1130299. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phillips D. R., Morrison M. The arrangement of proteins in the human erythrocyte membrane. Biochem Biophys Res Commun. 1970 Jul 27;40(2):284–289. doi: 10.1016/0006-291x(70)91007-7. [DOI] [PubMed] [Google Scholar]
- Sefton B. M., Wickus G. G., Burge B. W. Enzymatic iodination of Sindbis virus proteins. J Virol. 1973 May;11(5):730–735. doi: 10.1128/jvi.11.5.730-735.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanley P., Haslam E. A. The polypeptides of influenza virus. V. Localization of polypeptides in the virion by iodination techniques. Virology. 1971 Dec;46(3):764–773. doi: 10.1016/0042-6822(71)90078-x. [DOI] [PubMed] [Google Scholar]
- Uhr J. W., Vitetta E. S. Synthesis, biochemistry and dynamics of cell surface immunoglobulin on lymphocytes. Fed Proc. 1973 Jan;32(1):35–40. [PubMed] [Google Scholar]
- Vogt V. M., Eisenman R. Identification of a large polypeptide precursor of avian oncornavirus proteins. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1734–1738. doi: 10.1073/pnas.70.6.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]