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. 1974 Jan;13(1):62–72. doi: 10.1128/jvi.13.1.62-72.1974

Translation of Vesicular Stomatitis Messenger RNA by Extracts from Mammalian and Plant Cells

Trudy Morrison 1, Martha Stampfer 1, David Baltimore 1, Harvey F Lodish 1
PMCID: PMC355259  PMID: 4359431

Abstract

RNA was isolated from polyribosomes of vesicular stomatitis virus (VSV)-infected cells and tested for its ability to direct protein synthesis in extracts of animal and plant cells. In cell-free, non-preincubated extracts of rabbit reticulocytes, the 28S VSV RNA stimulated synthesis of a protein the size of the vesicular stomatitis virus L protein whereas the 13 to 15S RNA directed synthesis of the VSV M, N, NS, and possibly G proteins. In wheat germ extracts, 13 to 15S RNA also directed synthesis of the N, NS, M, and possibly G proteins. Analysis of extracts labeled with formyl [35S]methionine showed that the 28S RNA directed the initiation of synthesis of one protein, whereas the 13 to 15S RNA directed initiation of at least four proteins. It is concluded that the 28S RNA encodes only the L protein, whereas the 13 to 15S RNA is a mixture of species, presumably monocistronic, which code for the four other known vesicular stomatitis virus proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown F., Martin S. J., Cartwright B., Crick J. The ribonucleic acids of the infective and interfering components of vesicular stomatitis virus. J Gen Virol. 1967 Oct;1(4):479–486. doi: 10.1099/0022-1317-1-4-479. [DOI] [PubMed] [Google Scholar]
  2. Cartwright B., Talbot P., Brown F. The proteins of biologically active sub-units of vesicular stomatitis virus. J Gen Virol. 1970 Jun;7(3):267–272. doi: 10.1099/0022-1317-7-3-267. [DOI] [PubMed] [Google Scholar]
  3. Grubman M. J., Summers D. F. In vitro protein-synthesizing activity of vesicular stomatitis virus-infected cell extracts. J Virol. 1973 Aug;12(2):265–274. doi: 10.1128/jvi.12.2.265-274.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Housman D., Jacobs-Lorena M., Rajbhandary U. L., Lodish H. F. Initiation of haemoglobin synthesis by methionyl-tRNA. Nature. 1970 Aug 29;227(5261):913–918. doi: 10.1038/227913a0. [DOI] [PubMed] [Google Scholar]
  5. Huang A. S., Baltimore D., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus. 3. Multiple complementary messenger RNA molecules. Virology. 1970 Dec;42(4):946–957. doi: 10.1016/0042-6822(70)90343-0. [DOI] [PubMed] [Google Scholar]
  6. Huang A. S., Greenawalt J. W., Wagner R. R. Defective T particles of vesicular stomatitis virus. I. Preparation, morphology, and some biologic properties. Virology. 1966 Oct;30(2):161–172. doi: 10.1016/0042-6822(66)90092-4. [DOI] [PubMed] [Google Scholar]
  7. Huang A. S., Manders E. K. Ribonucleic acid synthesis of vesicular stomatitis virus. IV. Transcription by standard virus in the presence of defective interfering particles. J Virol. 1972 Jun;9(6):909–916. doi: 10.1128/jvi.9.6.909-916.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huang A. S., Wagner R. R. Comparative sedimentation coefficients of RNA extracted from plaque-forming and defective particles of vesicular stomatitis virus. J Mol Biol. 1966 Dec 28;22(2):381–384. doi: 10.1016/0022-2836(66)90143-4. [DOI] [PubMed] [Google Scholar]
  9. Huang A. S., Wagner R. R. Inhibition of cellular RNA synthesis by nonreplicating vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1579–1584. doi: 10.1073/pnas.54.6.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kang C. Y., Prevec L. Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens. J Virol. 1969 Apr;3(4):404–413. doi: 10.1128/jvi.3.4.404-413.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kingsbury D. W. Cell-free translation of paramyxovirus messenger RNA. J Virol. 1973 Nov;12(5):1020–1027. doi: 10.1128/jvi.12.5.1020-1027.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lodish H. F. Bacteriophage f2 RNA: control of translation and gene order. Nature. 1968 Oct 26;220(5165):345–350. doi: 10.1038/220345a0. [DOI] [PubMed] [Google Scholar]
  13. Lodish H. F. Biosynthesis of reticulocyte membrane proteins by membrane-free polyribosomes. Proc Natl Acad Sci U S A. 1973 May;70(5):1526–1530. doi: 10.1073/pnas.70.5.1526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McDowell M. J., Joklik W. K. An in vitro protein synthesizing system from mouse L fibroblasts infected with reovirus. Virology. 1971 Sep;45(3):724–733. doi: 10.1016/0042-6822(71)90186-3. [DOI] [PubMed] [Google Scholar]
  15. McDowell M. J., Joklik W. K., Villa-Komaroff L., Lodish H. F. Translation of reovirus messenger RNAs synthetesized in vitro into reovirus polypeptides by several mammalian cell-free extracts. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2649–2653. doi: 10.1073/pnas.69.9.2649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Moss B., Rosenblum E. N. Hydroxylapatite chromatography of protein-sodium dodecyl sulfate complexes. A new method for the separation of polypeptide subunits. J Biol Chem. 1972 Aug 25;247(16):5194–5198. [PubMed] [Google Scholar]
  17. Mudd J. A., Summers D. F. Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Oct;42(2):328–340. doi: 10.1016/0042-6822(70)90277-1. [DOI] [PubMed] [Google Scholar]
  18. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schincariol A. L., Howatson A. F. Replication of vesicular stomatitis virus. II. Separation and characterization of virus-specific RNA species. Virology. 1972 Sep;49(3):766–783. doi: 10.1016/0042-6822(72)90533-8. [DOI] [PubMed] [Google Scholar]
  20. Smith A. E. The initiation of protein synthesis directed by the RNA from encephalomyocarditis virus. Eur J Biochem. 1973 Mar 1;33(2):301–313. doi: 10.1111/j.1432-1033.1973.tb02684.x. [DOI] [PubMed] [Google Scholar]
  21. Stampfer M., Baltimore D., Huang A. S. Absence of interference during high-multiplicity infection by clonally purified vesicular stomatitis virus. J Virol. 1971 Mar;7(3):409–411. doi: 10.1128/jvi.7.3.409-411.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stampfer M., Baltimore D., Huang A. S. Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of ribonucleic acid found in Chinese hamster ovary cells infected with plaque-forming and defective particles. J Virol. 1969 Aug;4(2):154–161. doi: 10.1128/jvi.4.2.154-161.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stampfer M., Baltimore D. Identification of the vesicular stomatitis virus large protein as a unique viral protein. J Virol. 1973 Apr;11(4):520–526. doi: 10.1128/jvi.11.4.520-526.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WAGNER R. R., LEVEY A. H., SNYDER R. M., RATCLIFF G. A., Jr, HYATT D. F. BIOLOGIC PROPERTIES OF TWO PLAQUE VARIANTS OF VESICULAR STOMATITIS VIRUS (INDIANA SEROTYPE). J Immunol. 1963 Jul;91:112–122. [PubMed] [Google Scholar]
  25. Wagner R. R., Schnaitman T. C., Snyder R. M., Schnaitman C. A. Protein composition of the structural components of vesicular stomatitis virus. J Virol. 1969 Jun;3(6):611–618. doi: 10.1128/jvi.3.6.611-618.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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