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. 1974 Feb;13(2):253–260. doi: 10.1128/jvi.13.2.253-260.1974

Newcastle Disease Virus-Specific RNA: Polyacrylamide Gel Analysis of Single-Stranded RNA and Hybrid Duplexes

Nicolai V Kaverin 1, Natalia L Varich 1
PMCID: PMC355291  PMID: 4855736

Abstract

Newcastle disease virus-specific [3H]uridine-labeled 18S RNA was resolved by polyacrylamide gel electrophoresis into several components with molecular weights from 450,000 to 840,000. The analysis of 35 and 24S virus-specific RNA also revealed several components in each sedimentational class. The conversion of 18S RNA into double-stranded form by hybridization with an excess of unlabeled virion RNA improved the resolution in polyacrylamide gels and revealed at least six distinct components. The same six classes of hybrid duplexes were revealed when 32P-labeled 50S virion RNA was hybridized with an excess of 18S RNA. The applicability of polyacrylamide gel electrophoresis of hybrid duplexes to the analysis of viral genome structure is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blair C. D., Robinson W. S. Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology. 1968 Aug;35(4):537–549. doi: 10.1016/0042-6822(68)90284-5. [DOI] [PubMed] [Google Scholar]
  2. Collins B. S., Bratt M. A. Separation of the messenger RNAs of Newcastle disease virus by gel electrophoresis. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2544–2548. doi: 10.1073/pnas.70.9.2544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Compans R. W., Choppin P. W. The nucleic acid of the parainfluenza virus SV5. Virology. 1968 Jun;35(2):289–296. doi: 10.1016/0042-6822(68)90269-9. [DOI] [PubMed] [Google Scholar]
  4. Duesberg P. H. Physical properties of Rous Sarcoma Virus RNA. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1511–1518. doi: 10.1073/pnas.60.4.1511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  6. Ito Y., Joklik W. K. Temperature-sensitive mutants of reovirus. I. Patterns of gene expression by mutants of groups C, D, and E. Virology. 1972 Oct;50(1):189–201. doi: 10.1016/0042-6822(72)90359-5. [DOI] [PubMed] [Google Scholar]
  7. Kaverin N. V., Varich N. L. Sedimentation and hybridization analysis of virus-specific RNA in the course of Newcastle disease virus replication cycle. Arch Gesamte Virusforsch. 1972;38(2):183–191. doi: 10.1007/BF01249669. [DOI] [PubMed] [Google Scholar]
  8. Kingsbury D. W. Newcastle disease virus RNA. I. Isolation and preliminary characterization of RNA from virus particles. J Mol Biol. 1966 Jun;18(1):195–203. doi: 10.1016/s0022-2836(66)80085-2. [DOI] [PubMed] [Google Scholar]
  9. Kingsbury D. W. Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. J Mol Biol. 1966 Jun;18(1):204–214. doi: 10.1016/s0022-2836(66)80086-4. [DOI] [PubMed] [Google Scholar]
  10. Kingsbury D. W., Portner A., Darlington R. W. Properties of incomplete Sendai virions and subgenomic viral RNAs. Virology. 1970 Dec;42(4):857–871. doi: 10.1016/0042-6822(70)90335-1. [DOI] [PubMed] [Google Scholar]
  11. Lomniczi B., Meager A., Burke D. C. Virus RNA and protein synthesis in cells infected with different strains of Newcastle disease virus. J Gen Virol. 1971 Oct;13(1):111–120. doi: 10.1099/0022-1317-13-1-111. [DOI] [PubMed] [Google Scholar]
  12. Mountcastle W. E., Compans R. W., Choppin P. W. Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1971 Jan;7(1):47–52. doi: 10.1128/jvi.7.1.47-52.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
  14. Petermann M. L., Pavlovec A. The subunits and structural ribonucleic acids of Jensen sarcoma ribosomes. Biochim Biophys Acta. 1966 Feb 21;114(2):264–276. doi: 10.1016/0005-2787(66)90308-x. [DOI] [PubMed] [Google Scholar]
  15. Portner A., Kingsbury D. W. Complementary RNAs in paramyxovirions and paramyxovirus-infected cells. Nature. 1970 Dec 19;228(5277):1196–1197. doi: 10.1038/2281196a0. [DOI] [PubMed] [Google Scholar]
  16. Pridgen C., Kingsbury D. W. Adenylate-rich sequences in Sendai virus transcripts from infected cells. J Virol. 1972 Aug;10(2):314–317. doi: 10.1128/jvi.10.2.314-317.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robinson W. S. Self-annealing of subgroup 2 myxovirus RNAs. Nature. 1970 Mar 7;225(5236):944–945. doi: 10.1038/225944a0. [DOI] [PubMed] [Google Scholar]
  18. Schincariol A. L., Howatson A. F. Replication of vesicular stomatitis virus. II. Separation and characterization of virus-specific RNA species. Virology. 1972 Sep;49(3):766–783. doi: 10.1016/0042-6822(72)90533-8. [DOI] [PubMed] [Google Scholar]
  19. Wild T. F. Replication of vesicular stomatitis virus: characterization of the virus-induced RNA. J Gen Virol. 1971 Nov;13(2):295–310. doi: 10.1099/0022-1317-13-2-295. [DOI] [PubMed] [Google Scholar]

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