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. 1974 Feb;13(2):261–268. doi: 10.1128/jvi.13.2.261-268.1974

Molecular Weight Determination of Sendai and Newcastle Disease Virus RNA

Daniel Kolakofsky 1,2, Edward Boy de la Tour 1,2, Hajo Delius 1,2
PMCID: PMC355292  PMID: 4359293

Abstract

The molecular weights of Sendai and Newcastle disease virus RNA were estimated by sedimentation in sucrose gradients and by length measurements in the electron microscope under both denaturing and nondenaturing conditions. Sedimentation analyses under denaturing conditions yielded molecular weight estimates of 2.3 × 106 to 2.6 × 106, whereas length measurements yielded estimates of 5.2 × 106 to 5.6 × 106 for both denatured and nondenatured viral RNA. It would appear that the conditions of denaturation used (99% dimethyl sulfoxide at 26 C, and reaction with 1.1 M formaldehyde for 10 min at 60 C) do not equally denature parainfluenza virus RNA and other RNAs, such as cellular rRNA, 45S rRNA precursor, and R17 RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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