Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1974 Feb;13(2):285–290. doi: 10.1128/jvi.13.2.285-290.1974

Interactions of Polyoma and Mouse DNAs II. Polyoma-Induced Mouse DNA Replication and Pseudovirion Formation

Hans Türler 1
PMCID: PMC355295  PMID: 4359295

Abstract

Secondary mouse embryo (ME) cultures which had been grown prior to infection in the presence of 5-bromodeoxyuridine (BUdR) and 5-fluorodeoxy-uridine were found to be permissive for polyoma virus (16). The DNA extracted from the progeny virus yielded two bands on CsCl isopycnic centrifugation. The light band (LL) contained supercoiled circular (polyoma DNA I), open circular (polyoma DNA II), and linear (polyoma DNA III) molecules, as was seen by electron microscopy. The hybrid band (HL) contained exclusively linear molecules. This DNA was pure, density - labeled, pseudovirion DNA, i.e., fragmented HL mouse DNA. The quantitative comparison of HL and LL polyoma DNA III from six different virus preparations always revealed an excess of HL DNA, the ratio of HL/LL being between 1.2 and 2.2. These results led to the conclusion that in BUdR-prelabeled, polyoma-infected ME cells pseudovirion DNA is excised both from unreplicated and newly replicated regions of mouse DNA.

Full text

PDF
285

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Porat T., Kaplan A. S. Correlation between replication and degradation of cellular DNA in polyoma virus-infected cells. Virology. 1967 Jul;32(3):457–464. doi: 10.1016/0042-6822(67)90297-8. [DOI] [PubMed] [Google Scholar]
  2. DULBECCO R., HARTWELL L. H., VOGT M. INDUCTION OF CELLULAR DNA SYNTHESIS BY POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Feb;53:403–410. doi: 10.1073/pnas.53.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Friedmann T., Roblin R. Gene therapy for human genetic disease? Science. 1972 Mar 3;175(4025):949–955. doi: 10.1126/science.175.4025.949. [DOI] [PubMed] [Google Scholar]
  4. Grady L., Axelrod D., Trilling D. The SV40 pseudovirus: its potential for general transduction in animal cells. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1886–1893. doi: 10.1073/pnas.67.4.1886. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hirt B. Evidence for semiconservative replication of circular polyoma DNA. Proc Natl Acad Sci U S A. 1966 Apr;55(4):997–1004. doi: 10.1073/pnas.55.4.997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hirt B. Replicating molecules of polyoma virus DNA. J Mol Biol. 1969 Feb 28;40(1):141–144. doi: 10.1016/0022-2836(69)90302-7. [DOI] [PubMed] [Google Scholar]
  7. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  8. Kasamaki A., Ben-Porat T., Kaplan A. S. Polyoma virus-induced release of inhibition of cellular DNA synthesis caused by iododeoxyuridine. Nature. 1968 Feb 24;217(5130):756–758. doi: 10.1038/217756a0. [DOI] [PubMed] [Google Scholar]
  9. Le Pecq J. B., Paoletti C. A new fluorometric method for RNA and DNA determination. Anal Biochem. 1966 Oct;17(1):100–107. doi: 10.1016/0003-2697(66)90012-1. [DOI] [PubMed] [Google Scholar]
  10. Levine A. J., Teresky A. K. Deoxyribonucleic acid replication in simian virus 40-infected cells. II. Detection and characterization of simian virus 40 pseudovirions. J Virol. 1970 Apr;5(4):451–457. doi: 10.1128/jvi.5.4.451-457.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Michel M. R., Hirt B., Weil R. Mouse cellular DNA enclosed in polyoma viral capsids (pseudovirions). Proc Natl Acad Sci U S A. 1967 Oct;58(4):1381–1388. doi: 10.1073/pnas.58.4.1381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Qasba P. K., Aposhian H. V. DNA and gene therapy: transfer of mouse DNA to human and mouse embryonic cells by polyoma pseudovirions. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2345–2349. doi: 10.1073/pnas.68.10.2345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Trilling D. M., Axelrod D. Encapsidation of free host DNA by simian virus 40: a simian virus 40 pseudovirus. Science. 1970 Apr 10;168(3928):268–271. doi: 10.1126/science.168.3928.268. [DOI] [PubMed] [Google Scholar]
  15. Türler H. Interactions of polyoma and mouse DNAs. I. Lytic infection of bromodeoxyuridine-prelabeled mouse embryo cells. J Virol. 1974 Feb;13(2):276–284. doi: 10.1128/jvi.13.2.276-284.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WEIL R., VINOGRAD J. THE CYCLIC HELIX AND CYCLIC COIL FORMS OF POLYOMA VIRAL DNA. Proc Natl Acad Sci U S A. 1963 Oct;50:730–738. doi: 10.1073/pnas.50.4.730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WINOCOUR E. Purification of polyoma virus. Virology. 1963 Feb;19:158–168. doi: 10.1016/0042-6822(63)90005-9. [DOI] [PubMed] [Google Scholar]
  18. Weil R., Michel M. R., Ruschmann G. K. Induction of cellular DNA synthesis by polyoma virus. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1468–1475. doi: 10.1073/pnas.53.6.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Winocour E. Further studies on the incorporation of cell DNA into polyoma-related particles. Virology. 1968 Apr;34(4):571–582. doi: 10.1016/0042-6822(68)90078-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES