Abstract
DNA-RNA hybridization studies between 70S RNA from avian myeloblastosis virus (AMV) and an excess of DNA from (i) AMV-induced leukemic chicken myeloblasts or (ii) a mixture of normal and of congenitally infected K-137 chicken embryos producing avian leukosis viruses revealed the presence of fast- and slow-hybridizing virus-specific DNA sequences. However, the leukemic cells contained twice the level of AMV-specific DNA sequences observed in normal chicken embryonic cells. The fast-reacting sequences were two to three times more numerous in leukemic DNA than in DNA from the mixed embryos. The slow-reacting sequences had a reiteration frequency of approximately 9 and 6, in the two respective systems. Both the fast- and the slow-reacting DNA sequences in leukemic cells exhibited a higher Tm (2 C) than the respective DNA sequences in normal cells. In normal and leukemic cells the slow hybrid sequences appeared to have a Tm which was 2 C higher than that of the fast hybrid sequences. Individual non-virus-producing chicken embryos, either group-specific antigen positive or negative, contained 40 to 100 copies of the fast sequences and 2 to 6 copies of the slowly hybridizing sequences per cell genome. Normal rat cells did not contain DNA that hybridized with AMV RNA, whereas non-virus-producing rat cells transformed by B-77 avian sarcoma virus contained only the slowly reacting sequences. The results demonstrate that leukemic cells transformed by AMV contain new AMV-specific DNA sequences which were not present before infection.
Full text
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Altaner C., Temin H. M. Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitro by avian sarcoma viruses. Virology. 1970 Jan;40(1):118–134. doi: 10.1016/0042-6822(70)90384-3. [DOI] [PubMed] [Google Scholar]
- Baluda M. A., Drohan W. N. Distribution of deoxyribonucleic acid complementary to the ribonucleic acid of avian myeloblastosis virus in tissues of normal and tumor-bearing chickens. J Virol. 1972 Nov;10(5):1002–1009. doi: 10.1128/jvi.10.5.1002-1009.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baluda M. A., Nayak D. P. DNA complementary to viral RNA in leukemic cells induced by avian myeloblastosis virus. Proc Natl Acad Sci U S A. 1970 Jun;66(2):329–336. doi: 10.1073/pnas.66.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baluda M. A., Nayak D. P. Incorporation of precursors into ribonucleic acid, protein, glycoprotein, and lipoprotein of avian myeloblastosis virions. J Virol. 1969 Nov;4(5):554–566. doi: 10.1128/jvi.4.5.554-566.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baluda M. A. The role of the bursa-dependent lymphoid tissue in oncogenesis by avian myeloblastosis virus. Virology. 1967 Jul;32(3):428–437. doi: 10.1016/0042-6822(67)90294-2. [DOI] [PubMed] [Google Scholar]
- Baluda M. A. Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses. Proc Natl Acad Sci U S A. 1972 Mar;69(3):576–580. doi: 10.1073/pnas.69.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bishop J. O. DNA-RNA hybridization. Acta Endocrinol Suppl (Copenh) 1972;168:247–276. doi: 10.1530/acta.0.071s247. [DOI] [PubMed] [Google Scholar]
- Bishop J. O. Molecular hybridization of ribonucleic acid with a large excess of deoxyribonucleic acid. Biochem J. 1972 Jan;126(1):171–185. doi: 10.1042/bj1260171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
- Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
- CHAMBERLIN M., BERG P. MECHANISM OF RNA POLYMERASE ACTION: FORMATION OF DNA-RNA HYBRIDS WITH SINGLE-STRANDED TEMPLATES. J Mol Biol. 1964 Feb;8:297–313. doi: 10.1016/s0022-2836(64)80139-x. [DOI] [PubMed] [Google Scholar]
- Church R. B., Brown I. R. Tissue specificity of genetic transcription. Results Probl Cell Differ. 1972;3:11–24. doi: 10.1007/978-3-540-37149-6_2. [DOI] [PubMed] [Google Scholar]
- Coffin J. M. Rescue of rous sarcoma virus from rous sarcoma virus-transformed mammalian cells. J Virol. 1972 Jul;10(1):153–156. doi: 10.1128/jvi.10.1.153-156.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gelderman A. H., Rake A. V., Britten R. J. Transcription of nonrepeated DNA in neonatal and fetal mice. Proc Natl Acad Sci U S A. 1971 Jan;68(1):172–176. doi: 10.1073/pnas.68.1.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanafusa T., Hanafusa H. Isolation of leukosis-type virus from pheasant embryo cells: possible presence of viral genes in cells. Virology. 1973 Jan;51(1):247–251. doi: 10.1016/0042-6822(73)90388-7. [DOI] [PubMed] [Google Scholar]
- Harel L., Harel J., Frezouls G. DNA copies of viral RNA in rat cells transformed by Rous sarcoma virus (RSV). Biochem Biophys Res Commun. 1972 Aug 21;48(4):796–801. doi: 10.1016/0006-291x(72)90677-8. [DOI] [PubMed] [Google Scholar]
- Hayward W. S., Hanafusa H. Detection of avian tumor virus RNA in uninfected chicken embryo cells. J Virol. 1973 Feb;11(2):157–167. doi: 10.1128/jvi.11.2.157-167.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laird C. D. Chromatid structure: relationship between DNA content and nucleotide sequence diversity. Chromosoma. 1971 Mar 16;32(4):378–406. doi: 10.1007/BF00285251. [DOI] [PubMed] [Google Scholar]
- MCCARTHY B. J., BOLTON E. T. INTERACTION OF COMPLEMENTARY RNA AND DNA. J Mol Biol. 1964 Feb;8:184–200. doi: 10.1016/s0022-2836(64)80128-5. [DOI] [PubMed] [Google Scholar]
- McCarthy B. J., Church R. B. The specificity of molecular hybridization reactions. Annu Rev Biochem. 1970;39:131–150. doi: 10.1146/annurev.bi.39.070170.001023. [DOI] [PubMed] [Google Scholar]
- Melli M., Whitfield C., Rao K. V., Richardson M., Bishop J. O. DNA-RNA hybridization in vast DNA excess. Nat New Biol. 1971 May 5;231(18):8–12. [PubMed] [Google Scholar]
- Neiman P. E. Measurement of endogenous leukosis virus nucleotide sequences in the DNA of normal avian embryos by RNA-DNA hybridization. Virology. 1973 May;53(1):196–203. doi: 10.1016/0042-6822(73)90478-9. [DOI] [PubMed] [Google Scholar]
- Neiman P. E. Rous sarcoma virus nucleotide sequences in cellular DNA: measurement by RNA-DNA hybridization. Science. 1972 Nov 17;178(4062):750–753. doi: 10.1126/science.178.4062.750. [DOI] [PubMed] [Google Scholar]
- Niyogi S. K. The influence of chain length and base composition on the specific association of oligoribonucleotides with denatured deoxyribonucleic acid. J Biol Chem. 1969 Mar 25;244(6):1576–1581. [PubMed] [Google Scholar]
- Robinson W. S., Baluda M. A. The nucleic acid from avian myeloblastosis virus compared with the RNA from the Bryan strain of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1686–1692. doi: 10.1073/pnas.54.6.1686. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenthal P. N., Robinson H. L., Robinson W. S., Hanafusa T., Hanafusa H. DNA in uninfected and virus-infected cells complementary to avian tumor virus RNA. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2336–2340. doi: 10.1073/pnas.68.10.2336. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SINSHEIMER R. L., LAWRENCE M. IN VITRO SYNTHESIS AND PROPERTIES OF A PHI-X DNA-RNA HYBRID. J Mol Biol. 1964 Feb;8:289–296. doi: 10.1016/s0022-2836(64)80138-8. [DOI] [PubMed] [Google Scholar]
- STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
- SVOBODA J. Presence of chicken tumour virus in the sarcoma of the adult rat inoculated after birth with Rous sarcoma tissue. Nature. 1960 Jun 18;186:980–981. doi: 10.1038/186980b0. [DOI] [PubMed] [Google Scholar]
- Temin H. M. Mechanism of cell transformation by RNA tumor viruses. Annu Rev Microbiol. 1971;25:609–648. doi: 10.1146/annurev.mi.25.100171.003141. [DOI] [PubMed] [Google Scholar]
- Varmus H. E., Bishop J. M., Vogt P. K. Appearance of virus-specific DNA in mammalian cells following transformation by Rous sarcoma virus. J Mol Biol. 1973 Mar 15;74(4):613–626. doi: 10.1016/0022-2836(73)90052-1. [DOI] [PubMed] [Google Scholar]
- Weiss R. A., Friis R. R., Katz E., Vogt P. K. Induction of avian tumor viruses in normal cells by physical and chemical carcinogens. Virology. 1971 Dec;46(3):920–938. doi: 10.1016/0042-6822(71)90091-2. [DOI] [PubMed] [Google Scholar]
- Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]