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. 1974 Feb;13(2):394–399. doi: 10.1128/jvi.13.2.394-399.1974

Virus Replication in Enucleate Cells: Vesicular Stomatitis Virus and Influenza Virus

E A C Follett 1, C R Pringle 1, W H Wunner 1, J J Skehel 1
PMCID: PMC355309  PMID: 4359299

Abstract

The requirement of the presence of a nucleus for the replication of vesicular stomatitis virus and influenza virus has been examined by following the growth and development of these viruses in enucleate BS-C-1 cells. Vesicular stomatitis virus replicates normally in enucleate cells with the rate of production of infectious virus, the amount of virus-specific protein synthesis, and the type of proteins produced being essentially the same in nucleate and enucleate cells. Influenza virus does not replicate in enucleate cells, no virus gene products can be detected, and there is no inhibition of cellular protein synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARRY R. D., IVES D. R., CRUICKSHANK J. G. Participation of deoxyribonucleic acid in the multiplication of influenza virus. Nature. 1962 Jun 23;194:1139–1140. doi: 10.1038/1941139a0. [DOI] [PubMed] [Google Scholar]
  2. Carter S. B. Effects of cytochalasins on mammalian cells. Nature. 1967 Jan 21;213(5073):261–264. doi: 10.1038/213261a0. [DOI] [PubMed] [Google Scholar]
  3. Croce C. M., Koprowski H. Enucleation of cells made simple and rescue of SV40 by enucleated cells made even simpler. Virology. 1973 Jan;51(1):227–229. doi: 10.1016/0042-6822(73)90382-6. [DOI] [PubMed] [Google Scholar]
  4. Goldman R. D., Pollack R., Hopkins N. H. Preservation of normal behavior by enucleated cells in culture. Proc Natl Acad Sci U S A. 1973 Mar;70(3):750–754. doi: 10.1073/pnas.70.3.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HOPPS H. E., BERNHEIM B. C., NISALAK A., TJIO J. H., SMADEL J. E. BIOLOGIC CHARACTERISTICS OF A CONTINUOUS KIDNEY CELL LINE DERIVED FROM THE AFRICAN GREEN MONKEY. J Immunol. 1963 Sep;91:416–424. [PubMed] [Google Scholar]
  6. Pollack R., Goldman R. Synthesis of infective poliovirus in BSC-1 monkey cells enucleated with cytochalasin B. Science. 1973 Mar 2;179(4076):915–916. doi: 10.1126/science.179.4076.915. [DOI] [PubMed] [Google Scholar]
  7. Prescott D. M., Myerson D., Wallace J. Enucleation of mammalian cells with cytochalasin B. Exp Cell Res. 1972;71(2):480–485. doi: 10.1016/0014-4827(72)90322-9. [DOI] [PubMed] [Google Scholar]
  8. Scholtissek C., Rott R. Synthesis in vivo of influenza virus plus and minus strand RNA and its preferential inhibition by antibiotics. Virology. 1970 Apr;40(4):989–996. doi: 10.1016/0042-6822(70)90145-5. [DOI] [PubMed] [Google Scholar]
  9. Skehel J. J. Polypeptide synthesis in influenza virus-infected cells. Virology. 1972 Jul;49(1):23–36. doi: 10.1016/s0042-6822(72)80004-7. [DOI] [PubMed] [Google Scholar]
  10. Wagner R. R., Prevec L., Brown F., Summers D. F., Sokol F., MacLeod R. Classification of rhabdovirus proteins: a proposal. J Virol. 1972 Dec;10(6):1228–1230. doi: 10.1128/jvi.10.6.1228-1230.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wunner W. H., Pringle C. R. Protein synthesis in BHK21 cells infected with vesicular stomatitis virus. I. ts Mutants of the Indiana serotype. Virology. 1972 Apr;48(1):104–111. doi: 10.1016/0042-6822(72)90118-3. [DOI] [PubMed] [Google Scholar]

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