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. 1974 Feb;13(2):532–534. doi: 10.1128/jvi.13.2.532-534.1974

Proteinaceous Virus-like Particles from an Isolate of Aspergillus flavus

H Alan Wood 1,2, Robert F Bozarth 1,2, Jill Adler 1,2, Donald W Mackenzie 1,2,1
PMCID: PMC355325  PMID: 4129796

Abstract

Virus-like particles were purified from a single nonaflatoxin-producing isolate of Aspergillus flavus. The virus-like particles were spherical, measuring 27 to 30 nm in diameter, were electrophoretically homogeneous, and sedimented at approximately 49S. The particles had a buoyant density of 1.28 g/cm3 in CsCl and contained no detectable nucleic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRAKKE M. K. Photometric scanning of centrifuged density gradient columns. Anal Biochem. 1963 Apr;5:271–283. doi: 10.1016/0003-2697(63)90079-4. [DOI] [PubMed] [Google Scholar]
  2. Bozarth R. F., Wood H. A., Mandelbrot A. The Penicillium stoloniferum virus complex: two similar double-stranded RNA virus-like particles in a single cell. Virology. 1971 Aug;45(2):516–523. doi: 10.1016/0042-6822(71)90352-7. [DOI] [PubMed] [Google Scholar]
  3. Diener T. O., Schneider I. R. Virus degradation and nucleic acid release in single-phase phenol systems. Arch Biochem Biophys. 1968 Mar 20;124(1):401–412. doi: 10.1016/0003-9861(68)90344-5. [DOI] [PubMed] [Google Scholar]
  4. Dolman C. E., Chang E. Bacteriophages of Clostridium botulinum. Can J Microbiol. 1972 Jan;18(1):67–76. doi: 10.1139/m72-011. [DOI] [PubMed] [Google Scholar]
  5. FREEMAN V. J. Studies on the virulence of bacteriophage-infected strains of Corynebacterium diphtheriae. J Bacteriol. 1951 Jun;61(6):675–688. doi: 10.1128/jb.61.6.675-688.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Franklin R. M. Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504–1511. doi: 10.1073/pnas.55.6.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Loening U. E., Ingle J. Diversity of RNA components in green plant tissues. Nature. 1967 Jul 22;215(5099):363–367. doi: 10.1038/215363a0. [DOI] [PubMed] [Google Scholar]
  9. Wood H. A., Bozarth R. F., Mislivec P. B. Viruslike particles associated with an isolate of penicillium brevi-compactum. Virology. 1971 Jun;44(3):592–598. doi: 10.1016/0042-6822(71)90373-4. [DOI] [PubMed] [Google Scholar]
  10. Wood H. A., Bozarth R. F. Properties of viruslike particles of Penicillium chrysogenum: one double-stranded RNA molecule per particle. Virology. 1972 Mar;47(3):604–609. doi: 10.1016/0042-6822(72)90549-1. [DOI] [PubMed] [Google Scholar]
  11. Wood H. A. Buoyant density changes of cowpea mosaic virus components at different pH values. Virology. 1971 Feb;43(2):511–513. doi: 10.1016/0042-6822(71)90324-2. [DOI] [PubMed] [Google Scholar]
  12. Wood H. A., Streissle G. Wound tumor virus: purification and fractionation of the double-stranded ribonucleic acid. Virology. 1970 Feb;40(2):329–334. doi: 10.1016/0042-6822(70)90409-5. [DOI] [PubMed] [Google Scholar]

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