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. 1974 Mar;13(3):577–583. doi: 10.1128/jvi.13.3.577-583.1974

Polymerase Activity of Pichinde Virus

Michael F Carter a,1, Nilambar Biswal a, William E Rawls a,2
PMCID: PMC355340  PMID: 4132669

Abstract

Pichinde virus, a member of the arenavirus group, was examined for polymerase activity. Purified virus was found to contain RNA-dependent RNA polymerase but not RNA-dependent DNA polymerase activity. Since RNase but neither DNase nor actinomycin D inhibited the endogenous polymerase reaction, RNA of the virus appeared to be used as the template. The divalent cations Mg2+ and Mn2+ were required for optimal reactivity. The RNA product was partially resistant to RNase and the resistant portion had a sedimentation coefficient of 22 to 26S in sucrose gradients.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D. Expression of animal virus genomes. Bacteriol Rev. 1971 Sep;35(3):235–241. doi: 10.1128/br.35.3.235-241.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  4. Bishop D. H., Obijeski J. F., Simpson R. W. Transcription of the influenza ribonucleic acid genome by a virion polymerase. I. Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase. J Virol. 1971 Jul;8(1):66–73. doi: 10.1128/jvi.8.1.66-73.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Biswal N., Grizzard M. B., McCombs R. M., Benyesh-Melnick M. Characterization of intracellular ribonucleic acid specific for the murine sarcoma-leukemia virus complex. J Virol. 1968 Nov;2(11):1346–1352. doi: 10.1128/jvi.2.11.1346-1352.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Borsa J., Graham A. F. Reovirus: RNA polymerase activity in purified virions. Biochem Biophys Res Commun. 1968 Dec 30;33(6):895–901. doi: 10.1016/0006-291x(68)90396-3. [DOI] [PubMed] [Google Scholar]
  7. Carter M. F., Biswal N., Rawls W. E. Characterization of nucleic acid of pichinde virus. J Virol. 1973 Jan;11(1):61–68. doi: 10.1128/jvi.11.1.61-68.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Carter M. F., Murphy F. A., Brunschwig J. P., Noonan C., Rawls W. E. Effects of actinomycin D and ultraviolet and ionizing radiation on Pichinde virus. J Virol. 1973 Jul;12(1):33–38. doi: 10.1128/jvi.12.1.33-38.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chow N. L., Simpson R. W. RNA-dependent RNA polymerase activity associated with virions and subviral particles of myxoviruses. Proc Natl Acad Sci U S A. 1971 Apr;68(4):752–756. doi: 10.1073/pnas.68.4.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Farber F. E. Studies on RNA metabolism in Acetabularia mediterranea. I. The isolation of RNA and labelling studies on RNA of whole plants and plant fragments. Biochim Biophys Acta. 1969 Jan 21;174(1):1–11. [PubMed] [Google Scholar]
  11. Furman P. A., Hallum J. V. RNA-dependent DNA polymerase activity in preparations of a mutant of Newcastle disease virus arising from persistently infected L cells. J Virol. 1973 Sep;12(3):548–555. doi: 10.1128/jvi.12.3.548-555.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Groves W. E., Davis F. C., Jr, Sells B. H. Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. Anal Biochem. 1968 Feb;22(2):195–210. doi: 10.1016/0003-2697(68)90307-2. [DOI] [PubMed] [Google Scholar]
  13. Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hutchinson J. E., Mahy B. W. RNA polymerase activity in virions of parainfluenza 1 virus (Sendai). Arch Gesamte Virusforsch. 1972;37(2):203–210. doi: 10.1007/BF01268002. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Lin F. H., Thormar H. Properties of maedi nucleic acid and the presence of ribonucleic acid- and deoxyribonucleic acid-dependent deoxyribonucleic acid polymerase in the virions. J Virol. 1972 Aug;10(2):228–233. doi: 10.1128/jvi.10.2.228-233.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lin F. H., Thormar H. Ribonucleic acid-dependent deoxyribonucleic acid polymerase in visna virus. J Virol. 1970 Nov;6(5):702–704. doi: 10.1128/jvi.6.5.702-704.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  19. Mifune K., Carter M., Rawls W. Characterization studies of the Pichinde virus-a member of the arenavirus group. Proc Soc Exp Biol Med. 1971 Feb;136(2):637–644. doi: 10.3181/00379727-136-35330. [DOI] [PubMed] [Google Scholar]
  20. Robinson W. S. Ribonucleic acid polymerase activity in Sendai virions and nucleocapsid. J Virol. 1971 Jul;8(1):81–86. doi: 10.1128/jvi.8.1.81-86.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rowe W. P., Murphy F. A., Bergold G. H., Casals J., Hotchin J., Johnson K. M., Lehmann-Grube F., Mims C. A., Traub E., Webb P. A. Arenoviruses: proposed name for a newly defined virus group. J Virol. 1970 May;5(5):651–652. doi: 10.1128/jvi.5.5.651-652.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Scolnick E. M., Aaronson S. A., Todaro G. J. DNA synthesis by RNA-containing tumor viruses. Proc Natl Acad Sci U S A. 1970 Oct;67(2):1034–1041. doi: 10.1073/pnas.67.2.1034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shatkin A. J., Sipe J. D. RNA polymerase activity in purified reoviruses. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462–1469. doi: 10.1073/pnas.61.4.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Skehel J. J. RNA-dependent RNA polymerase activity of the influenza virus. Virology. 1971 Sep;45(3):793–796. doi: 10.1016/0042-6822(71)90197-8. [DOI] [PubMed] [Google Scholar]
  25. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]
  26. Trapido H., Sanmartín C. Pichindé virus, a new virus of the Tacaribe group from Colombia. Am J Trop Med Hyg. 1971 Jul;20(4):631–641. [PubMed] [Google Scholar]

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