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. 1974 Mar;13(3):699–705. doi: 10.1128/jvi.13.3.699-705.1974

Immunochemical Procedure for Partial Purification of Newly Synthesized Proteins in Polyoma Virus-Infected Mouse Cells

Denise Paulin 1, Jacqueline Perreau 1, François Cuzin 1
PMCID: PMC355356  PMID: 4362872

Abstract

A broad-spectrum antiserum has been obtained against nuclear proteins from normal mouse fibroblasts. Solid phase immunoadsorbants prepared from the serum specifically bind the corresponding antigens. The removal in this way of a significant fraction of host proteins leads to partial purification of viral and virus-induced polypeptides from polyoma virus-infected cell extracts. Four main peptides can be selected, with respective molecular weights of 90,000, 70,000, 46,000, and 41,000.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. W., Gesteland R. F. Pattern of protein synthesis in monkey cells infected by simian virus 40. J Virol. 1972 May;9(5):758–765. doi: 10.1128/jvi.9.5.758-765.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
  3. Cuatrecasas P. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970 Jun;245(12):3059–3065. [PubMed] [Google Scholar]
  4. Del Villano B. C., Defendi V. Characterization of the SV40 T antigen. Virology. 1973 Jan;51(1):34–46. doi: 10.1016/0042-6822(73)90363-2. [DOI] [PubMed] [Google Scholar]
  5. Fischer H., Sauer G. Identification of virus-induced proteins in cells productively infected with simian virus 40. J Virol. 1972 Jan;9(1):1–9. doi: 10.1128/jvi.9.1.1-9.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frearson P. M., Crawford L. V. Polyoma virus basic proteins. J Gen Virol. 1972 Feb;14(2):141–155. doi: 10.1099/0022-1317-14-2-141. [DOI] [PubMed] [Google Scholar]
  7. Friedmann T., David D. Structural roles of polyoma virus proteins. J Virol. 1972 Oct;10(4):776–782. doi: 10.1128/jvi.10.4.776-782.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Griffith I. P. Immediate visualization of proteins in dodecyl sulfate-polyacrylamide gels by prestaining with Remazol dyes. Anal Biochem. 1972 Apr;46(2):402–412. doi: 10.1016/0003-2697(72)90313-2. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Roblin R., Härle E., Dulbecco R. Polyoma virus proteins. 1. Multiple virion components. Virology. 1971 Sep;45(3):555–566. doi: 10.1016/0042-6822(71)90171-1. [DOI] [PubMed] [Google Scholar]
  11. Sambrook J. Transformation by polyoma virus and simian virus 40. Adv Cancer Res. 1972;16:141–180. [PubMed] [Google Scholar]
  12. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  13. VOGT M., DULBECCO R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology. 1962 Jan;16:41–51. doi: 10.1016/0042-6822(62)90200-3. [DOI] [PubMed] [Google Scholar]
  14. WINOCOUR E. Purification of polyoma virus. Virology. 1963 Feb;19:158–168. doi: 10.1016/0042-6822(63)90005-9. [DOI] [PubMed] [Google Scholar]
  15. Walter G., Roblin R., Dulbecco R. Protein synthesis in Simian virus 40-infected monkey cells. Proc Natl Acad Sci U S A. 1972 Apr;69(4):921–924. doi: 10.1073/pnas.69.4.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  17. Wicker R., Avrameas S. Localization of virus antigens by enzyme-labelled antibodies. J Gen Virol. 1969 Jun;4(4):465–471. doi: 10.1099/0022-1317-4-4-465. [DOI] [PubMed] [Google Scholar]

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