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. 1974 Mar;13(3):721–728. doi: 10.1128/jvi.13.3.721-728.1974

Replication of Measles Virus: Distinct Species of Short Nucleocapsids in Cytoplasmic Extracts of Infected Cells

Michael P Kiley 1, Robert H Gray 1, Francis E Payne 1
PMCID: PMC355359  PMID: 4132672

Abstract

Cytoplasmic extracts of Vero cells infected with wild-strain Edmonston measles virus were found to contain two and probably three distinct species of nucleocapsids. Species sedimenting at 200 and 110S contained RNA which sedimented at 50 and 16 to 18S, respectively. The third nucleocapsid species which sedimented at 170S was not present in all experiments and was not characterized in detail. Essentially all 200 and 170S, as well as a portion of the 110S, nucleocapsids were membrane associated and probably present in part in cell-associated virions. Five of six plaque purified strains derived from wild-type Edmonston virus produced only 200S nucleocapsids. One of these five plaque-purified strains subsequently produced both 200 and 110S nucleocapsids after being passaged by using undiluted inocula. These results suggest that measles virus may produce distinct classes of defective virus containing short nucleocapsids and subgenomic viral RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baublis J. V., Payne F. E. Measles antigen and syncytium formtion in brain cell cultures from subacute sclerosing panencephalitis (SSPE). Proc Soc Exp Biol Med. 1968 Nov;129(2):593–597. doi: 10.3181/00379727-129-33377. [DOI] [PubMed] [Google Scholar]
  2. Blair C. D., Robinson W. S. Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology. 1968 Aug;35(4):537–549. doi: 10.1016/0042-6822(68)90284-5. [DOI] [PubMed] [Google Scholar]
  3. Carter C., Schuluederberg A., Black F. L. Viral RNA synthesis in measles virus-infected cells. Virology. 1973 Jun;53(2):379–383. doi: 10.1016/0042-6822(73)90217-1. [DOI] [PubMed] [Google Scholar]
  4. East J. L., Kingsbury D. W. Mumps virus replication in chick embryo lung cells: properties of ribonucleic acids in virions and infected cells. J Virol. 1971 Aug;8(2):161–173. doi: 10.1128/jvi.8.2.161-173.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HOPPS H. E., BERNHEIM B. C., NISALAK A., TJIO J. H., SMADEL J. E. BIOLOGIC CHARACTERISTICS OF A CONTINUOUS KIDNEY CELL LINE DERIVED FROM THE AFRICAN GREEN MONKEY. J Immunol. 1963 Sep;91:416–424. [PubMed] [Google Scholar]
  6. Hall W. W., Martin S. J. Purification and characterization of measles virus. J Gen Virol. 1973 May;19(2):175–188. doi: 10.1099/0022-1317-19-2-175. [DOI] [PubMed] [Google Scholar]
  7. Huang A. S., Baltimore D. Defective viral particles and viral disease processes. Nature. 1970 Apr 25;226(5243):325–327. doi: 10.1038/226325a0. [DOI] [PubMed] [Google Scholar]
  8. Kiley M. P., Wagner R. R. Ribonucleic acid species of intracellular nucleocapsids and released virions of vesicular stomatitis virus. J Virol. 1972 Aug;10(2):244–255. doi: 10.1128/jvi.10.2.244-255.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kingsbury D. W., Portner A., Darlington R. W. Properties of incomplete Sendai virions and subgenomic viral RNAs. Virology. 1970 Dec;42(4):857–871. doi: 10.1016/0042-6822(70)90335-1. [DOI] [PubMed] [Google Scholar]
  10. Nakai T., Shand F. L., Howatson A. F. Development of measles virus in vitro. Virology. 1969 May;38(1):50–67. doi: 10.1016/0042-6822(69)90127-5. [DOI] [PubMed] [Google Scholar]
  11. Parfanovich M., Hammarskjöld B., Norrby E. Synthesis of virus-specific RNA in cells infected with two different variants of measles virus. Arch Gesamte Virusforsch. 1971;35(1):38–44. doi: 10.1007/BF01249750. [DOI] [PubMed] [Google Scholar]
  12. Payne F. E., Baublis J. V. Decreased reactivity of SSPE strains of measles virus with antibody. J Infect Dis. 1973 May;127(5):505–511. doi: 10.1093/infdis/127.5.505. [DOI] [PubMed] [Google Scholar]
  13. Payne F. E., Baublis J. V., Itabashi H. H. Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. N Engl J Med. 1969 Sep 11;281(11):585–589. doi: 10.1056/NEJM196909112811103. [DOI] [PubMed] [Google Scholar]
  14. Penman S., Scherrer K., Becker Y., Darnell J. E. POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA. Proc Natl Acad Sci U S A. 1963 May;49(5):654–662. doi: 10.1073/pnas.49.5.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schluederberg A. Measles virus RNA. Biochem Biophys Res Commun. 1971 Mar 19;42(6):1012–1015. doi: 10.1016/0006-291x(71)90004-0. [DOI] [PubMed] [Google Scholar]
  16. WATERSON A. P. Two kinds of myxovirus. Nature. 1962 Mar 24;193:1163–1164. doi: 10.1038/1931163a0. [DOI] [PubMed] [Google Scholar]
  17. Winston S. H., Rustigian R., Bratt M. A. Persistent infection of cells in culture by measles virus. 3. Comparison of virus-specific RNA synthesized in primary persistent infection in HeLa cells. J Virol. 1973 Jun;11(6):926–932. doi: 10.1128/jvi.11.6.926-932.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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