Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1974 Apr;13(4):801–808. doi: 10.1128/jvi.13.4.801-808.1974

Mode of Action of Acid pH Values on the Development of Vesicular Stomatitis Virus

Marc Fiszman 1,2, J-B Leaute 1,2, C Chany 1,2, Marc Girard 1,2
PMCID: PMC355378  PMID: 4132389

Abstract

The effect of low pH's on the development of vesicular stomatitis virus (VSV) was studied. L cells infected with VSV were incubated at pH 6.6. A 99% inhibition in the yield of infectious particles was observed by comparison with the yield at pH 7.4. Such inhibition was not due to inhibition of viral RNA synthesis, since at pH 6.6 all the known species of VSV RNA molecules were synthesized. Furthermore, all the known species of VSV proteins were also synthesized. However, no viral particles nor nucleocapsids were detected. Raising the pH to 6.9 resulted in the appearance of nucleocapsids and viral particles, although the yield of infectious virus was still inhibited by 90%. The lack of infectivity of these pH 6.9 viral particles was correlated with their inability to promote primary transcription. The hypothesis that low pH's alter the correct positioning of the viral proteins into the cell membrane is presented.

Full text

PDF
801

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fiszman M., Reynier M., Bucchini D., Girard M. Thermosensitive block of the Sabin strain of poliovirus type I. J Virol. 1972 Dec;10(6):1143–1151. doi: 10.1128/jvi.10.6.1143-1151.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Flamand A. Etude génétique du virus de la stomatite vésiculaire: classement de mutants thermosensibles spontanés en groupes de complémentation. J Gen Virol. 1970 Sep;8(3):187–195. doi: 10.1099/0022-1317-8-3-187. [DOI] [PubMed] [Google Scholar]
  4. Huang A. S., Manders E. K. Ribonucleic acid synthesis of vesicular stomatitis virus. IV. Transcription by standard virus in the presence of defective interfering particles. J Virol. 1972 Jun;9(6):909–916. doi: 10.1128/jvi.9.6.909-916.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LWOFF A., LWOFF M. [On factors of viral growth and their role in the development of infection]. Ann Inst Pasteur (Paris) 1960 Feb;98:173–203. [PubMed] [Google Scholar]
  6. Leaute J. B., Fiszman M. Y., Chany C., Girard M. Effet d'un abaissement de pH sur le développement du virus de la stomatite vésiculaire. C R Acad Sci Hebd Seances Acad Sci D. 1971 Nov 29;273(22):2176–2179. [PubMed] [Google Scholar]
  7. Léauté J. B., Fiszman M. Y., Chany C., Girard M. Etude de l'influence d'un abaissement de pH sur le métabolisme cellulaire, et sur le développement du virus de la poliomyélite. C R Acad Sci Hebd Seances Acad Sci D. 1971 Nov 22;273(21):2024–2027. [PubMed] [Google Scholar]
  8. Marcus P. I., Engelhardt D. L., Hunt J. M., Sekellick M. J. Interferon action: inhibition of vesicular stomatitis virus RNA synthesis induced by virion-bound polymerase. Science. 1971 Nov 5;174(4009):593–598. doi: 10.1126/science.174.4009.593. [DOI] [PubMed] [Google Scholar]
  9. Mudd J. A., Summers D. F. Polysomal ribonucleic acid of vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Dec;42(4):958–968. doi: 10.1016/0042-6822(70)90344-2. [DOI] [PubMed] [Google Scholar]
  10. Newman J. F., Brown F. Induced ribonucleic acids in cells infected with vesicular stomatitis virus. J Gen Virol. 1969 Sep;5(2):305–313. doi: 10.1099/0022-1317-5-2-305. [DOI] [PubMed] [Google Scholar]
  11. Printz P., Wagner R. R. Temperature-sensitive mutants of vesicular stomatitis virus: synthesis of virus-specific proteins. J Virol. 1971 May;7(5):651–662. doi: 10.1128/jvi.7.5.651-662.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Roy P., Bishop D. H. Genome homology of vesicular stomatitis virus and defective T particles and evidence for the sequential transcription of the virion ribonucleic acid. J Virol. 1972 Jun;9(6):946–955. doi: 10.1128/jvi.9.6.946-955.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schincariol A. L., Howatson A. F. Replication of vesicular stomatitis virus. I. Viral specific RNA and nucleoprotein in infected L cells. Virology. 1970 Nov;42(3):732–743. doi: 10.1016/0042-6822(70)90319-3. [DOI] [PubMed] [Google Scholar]
  14. Stampfer M., Baltimore D., Huang A. S. Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of ribonucleic acid found in Chinese hamster ovary cells infected with plaque-forming and defective particles. J Virol. 1969 Aug;4(2):154–161. doi: 10.1128/jvi.4.2.154-161.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wagner R. R., Schnaitman T. A., Snyder R. M. Structural proteins of vesicular stomatitis viruses. J Virol. 1969 Apr;3(4):395–403. doi: 10.1128/jvi.3.4.395-403.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wagner R. R., Schnaitman T. C., Snyder R. M., Schnaitman C. A. Protein composition of the structural components of vesicular stomatitis virus. J Virol. 1969 Jun;3(6):611–618. doi: 10.1128/jvi.3.6.611-618.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wagner R. R., Snyder R. M., Yamazaki S. Proteins of vesicular stomatitis virus: kinetics and cellular sites of synthesis. J Virol. 1970 May;5(5):548–558. doi: 10.1128/jvi.5.5.548-558.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wild T. F. Replication of vesicular stomatitis virus: characterization of the virus-induced RNA. J Gen Virol. 1971 Nov;13(2):295–310. doi: 10.1099/0022-1317-13-2-295. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES