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. 1974 Apr;13(4):828–836. doi: 10.1128/jvi.13.4.828-836.1974

Early Biochemical Events Occurring After Infection of Bacillus cereus 569-SP1 with Bacteriophage GSW

Hevion Ben-Horin a,1, M Antoinette Walz a,1, Arthur K Saz a
PMCID: PMC355381  PMID: 4206941

Abstract

After infection of Bacillus cereus 569-SP1 with the 5-hydroxymethyluracil-containing phage GSW, new dTTPase, dUTPase, and dUMP-hydroxymethylase activities appear. No significant changes in activities of other pyrimidine ribonucleoside or 2′-deoxyribonucleoside triphosphate nucleotidohydrolases were detected. dUTP and dUMP inhibit the dTTPase activity, whereas dTTP failed to inhibit dUTPase activity. The Km value for the substrate dUTP is 10−4 M and for dTTP is 4.85 × 10−4 M. Thymidylate synthetase activity is inhibited only when cells are infected during the late lag or very early log phases of growth; when cells are infected with phage during mid-log, thymidylate synthetase activity is unaffected. The data support the suggestion that, although phage GSW may inhibit an otherwise expected increase in activity of thymidylate synthetase, it fails to affect the already existing activity. The data presented do not allow discrimination as to whether the phage specifies inhibition of de novo synthesis of thymidylate synthetase or the increase in activity of already existing but not fully expressed enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dunlap R. B., Harding N. G., Huennekens F. M. Thymidylate synthetase from amethopterin-resistant Lactobacillus casei. Biochemistry. 1971 Jan 5;10(1):88–97. doi: 10.1021/bi00777a014. [DOI] [PubMed] [Google Scholar]
  2. FLAKS J. G., COHEN S. S. Virus-induced acquisition of metabolic function. III. Formation and some properties of thymidylate synthetase of bacteriophage-infected Escherichia coli. J Biol Chem. 1959 Nov;234:2981–2986. [PubMed] [Google Scholar]
  3. Greenberg G. R. New dUTPase and dUDPase activites after infection of Escherichia coli by T2 bacteriophage. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1226–1232. doi: 10.1073/pnas.56.4.1226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Nishihara M., Chrambach A., Aposhian H. V. The deoxycytidylate deaminase found in Bacillus subtilis infected with phage SP8. Biochemistry. 1967 Jul;6(7):1877–1886. doi: 10.1021/bi00859a001. [DOI] [PubMed] [Google Scholar]
  6. Price A. R., Dunham L. F., Walker R. L. Thymidine triphosphate nucleotidohydrolase and deoxyuridylate hydroxymethylase induced by mutants of Bacillus subtilis bacteriophage SP82G. J Virol. 1972 Dec;10(6):1240–1241. doi: 10.1128/jvi.10.6.1240-1241.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Roscoe D. H. Synthesis of DNA in phage-infected Bacillus subtilis. Virology. 1969 Aug;38(4):527–537. doi: 10.1016/0042-6822(69)90173-1. [DOI] [PubMed] [Google Scholar]
  8. Roscoe D. H. Thymidine triphosphate nucleotidohydrolase: a phage-induced enzyme in Bacillus subtilis. Virology. 1969 Aug;38(4):520–526. doi: 10.1016/0042-6822(69)90172-x. [DOI] [PubMed] [Google Scholar]
  9. Roscoe D. H., Tucker R. G. The biosynthesis of 5-hydroxymethyldeoxyuridylic acid in bacteriophage-infected Bacillus subtilis. Virology. 1966 May;29(1):157–166. doi: 10.1016/0042-6822(66)90205-4. [DOI] [PubMed] [Google Scholar]
  10. Sedmak J., Ramaley R. Purification and properties of Bacillus subtilis nucleoside diphosphokinase. J Biol Chem. 1971 Sep 10;246(17):5365–5372. [PubMed] [Google Scholar]
  11. Shen S. R., Schmidt R. R. Enzymic control of nucleic acid synthesis during synchronous growth of Chlorella pyrenoidosa. II. Deoxycytidine monophosphate deaminase. Arch Biochem Biophys. 1966 Jul;115(1):13–20. doi: 10.1016/s0003-9861(66)81031-7. [DOI] [PubMed] [Google Scholar]
  12. Thorne C. B. Transducing bacteriophage for Bacillus cereus. J Virol. 1968 Jul;2(7):657–662. doi: 10.1128/jvi.2.7.657-662.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WAHBA A. J., FRIEDKIN M. The enzymatic synthesis of thymidylate. I. Early steps in the purification of thymidylate synthetase of Escherichia coli. J Biol Chem. 1962 Dec;237:3794–3801. [PubMed] [Google Scholar]
  14. Warner H. R., Barnes J. E. Evidence for a dual role for the bacteriophage T4-induced deoxycytidine triphosphate nucleotidohydrolase. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1233–1240. doi: 10.1073/pnas.56.4.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ZIMMERMAN S. B., KORNBERG A. Deoxycytidine di- and triphosphate cleavage by an enzyme formed in bacteriophage-infected Eschrichia coli. J Biol Chem. 1961 May;236:1480–1486. [PubMed] [Google Scholar]

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