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. 1974 Apr;13(4):881–887. doi: 10.1128/jvi.13.4.881-887.1974

Effect of d-Penicillamine on Poliovirus Replication In HeLa Cells

Parvin Merryman 1, Israeli A Jaffe 1, Ellie Ehrenfeld 1
PMCID: PMC355386  PMID: 4362514

Abstract

A series of mercaptan compounds were studied with respect to their ability to inhibit the growth of poliovirus in cultured cells. Of the compounds tested only d-penicillamine possessed antiviral activity. There was no direct effect on the virus itself, nor were the processes of adsorption, penetration and uncoating, or virus-induced “shut-off” of host cell protein synthesis inhibited. At concentrations where there was no effect on host cell RNA or protein synthesis, d-penicillamine caused a marked inhibition of virus-specific RNA and protein synthesis. Although much reduced, the relative concentrations of single-stranded and double-stranded viral RNA synthesized in the presence of d-penicillamine was unchanged. Similarly, all apparent precursor and cleavage product proteins could be synthesized in the presence of the drug. The inhibitory effect was reversible, after a lag of 1.5 to 2 h after removal of the drug, and normal yields of virus could be obtained. The structural and functional properties of d-penicillamine are discussed in relation to requirements for anti-polioviral activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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