Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1974 May;13(5):1143–1147. doi: 10.1128/jvi.13.5.1143-1147.1974

Separation of B and C Type Virions by Centrifugation in Gentle Density Gradients

Nurul H Sarkar 1, Dan H Moore 1
PMCID: PMC355425  PMID: 4363254

Abstract

Murine type B particles were separated from type C (Rauscher leukemia virus) by means of gentle (low-increment rate) density gradients. The best separation was obtained when the density ranged from 1.13 to 1.20 g/cm3 when sucrose was used and from 1.12 to 1.28 g/cm3 with CsCl. The buoyant densities of the B and C particle bands in sucrose were 1.18 and 1.16 g/cm3, respectively. The CsCl gradient gave a better separation with the B particles banding at a density of 1.20 g/cm3 and with the C particle density little different from its value in sucrose.

Full text

PDF
1143

Images in this article

1144Image
on p.1144
1145Image
on p.1145

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dmochowski L., Williams W. C., Swearingen G. R., Myers B., Fujinaga S. Immunological studies on mouse mammary tumors and leukemia. Tex Rep Biol Med. 1971 Spring;29(1):41–62. [PubMed] [Google Scholar]
  2. Hardy W. D., Jr, Geering G., Old L. J., DE Harven E., Brody R. S., McDonough S. Feline leukemia virus: occurrence of viral antigen in the tissues of cats with lymphosarcoma and other diseases. Science. 1969 Nov 21;166(3908):1019–1021. doi: 10.1126/science.166.3908.1019. [DOI] [PubMed] [Google Scholar]
  3. Hilgers J., Williams W. C., Myers B., Dmochowski L. Detection of antigens of the mouse mammary tumor (MTV) and murine leukemia virus (MuLV) in cells of cultures derived from mammary tumors of mice of several strains. Virology. 1971 Aug;45(2):470–483. doi: 10.1016/0042-6822(71)90347-3. [DOI] [PubMed] [Google Scholar]
  4. Lasfargues E. Y., Kramarsky B., Sarkar N. H., Lasfargues J. C., Pillsbury N., Moore D. H. Stimulation of mammary tumor virus production in a mouse mammary tumor cell line. Cancer Res. 1970 Apr;30(4):1109–1117. [PubMed] [Google Scholar]
  5. McGrath C. M. Replication of mammary tumor virus in tumor cell cultures: dependence on hormone-induced cellular organization. J Natl Cancer Inst. 1971 Aug;47(2):455–467. [PubMed] [Google Scholar]
  6. Moore D. H., Charney J., Kramarsky B., Lasfargues E. Y., Sarkar N. H., Brennan M. J., Burrows J. H., Sirsat S. M., Paymaster J. C., Vaidya A. B. Search for a human breast cancer virus. Nature. 1971 Feb 26;229(5287):611–614. doi: 10.1038/229611a0. [DOI] [PubMed] [Google Scholar]
  7. Moore D. H., Sarkar N. H., Charney J., Kramarsky B. Some physical and biological characteristics of the mouse mammary tumor virus. Cancer Res. 1973 Jan;33(1):5–10. [PubMed] [Google Scholar]
  8. Nowinski R. C., Edynak E., Sarkar N. H. Serological and structural properties of Mason-Pfizer monkey virus isolated from the mammary tumor of a Rhesus monkey. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1608–1612. doi: 10.1073/pnas.68.7.1608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sarkar N. H., Moore D. H. Electron microscopy of the nucleic acid of mouse mammary tumor virus. J Virol. 1970 Feb;5(2):230–236. doi: 10.1128/jvi.5.2.230-236.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sarkar N. H., Moore D. H. On the possibility of a human breast cancer virus. Nature. 1972 Mar 17;236(5342):103–106. doi: 10.1038/236103a0. [DOI] [PubMed] [Google Scholar]
  11. Schlom J., Spiegelman S., Moore D. H. Reverse transcriptase and high molecular weight RNA in particles from mouse and human milk. J Natl Cancer Inst. 1972 Apr;48(4):1197–1203. [PubMed] [Google Scholar]
  12. Stromberg K. Surface-active agents for isolation of the core component of avian myeloblastosis virus. J Virol. 1972 Apr;9(4):684–697. doi: 10.1128/jvi.9.4.684-697.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES