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. 1974 Jun;13(6):1231–1236. doi: 10.1128/jvi.13.6.1231-1236.1974

DNA Polymerase in the Core of the Human Hepatitis B Virus Candidate

William S Robinson 1, Richard L Greenman 1
PMCID: PMC355442  PMID: 4833609

Abstract

Experiments were done to show that the human hepatitis B antigen (HBAg)-associated DNA polymerase is a component of Dane particles and their antigenically distinct cores prepared by Nonidet P-40 detergent treatment of Dane particles. Before detergent treatment, the DNA polymerase was precipitated by serum containing anti-HB surface antigen (anti-HBs) but not with serum containing anti-HB core antigen (anti-HBc). After detergent treatment, the enzyme was precipitated by anti-HBc- and not by anti-HBs-containing serum. Highly purified 16- to 25-nm HBAg particles blocked only the precipitation of DNA polymerase in untreated HBAg preparations. The 110S structure with which the DNA reaction product remains associated in Nonidet P-40-treated preparations was identified as Dane particle core by immunoprecipitation with serum containing anti-HBc. The DNA polymerase and the radioactive DNA reaction product were used as markers for core in immunoprecipitation tests for anticore. In such assays, 8 of 11 human sera with anti-HBs activity and all of 10 sera from chronic HBAg carriers were found to contain anti-HBc activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almeida J. D. Electron microscopic observations and speculations on Australia antigen. Postgrad Med J. 1971 Jul;47(549):484–487. doi: 10.1136/pgmj.47.549.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Almeida J. D., Rubenstein D., Stott E. J. New antigen-antibody system in Australia-antigen-positive hepatitis. Lancet. 1971 Dec 4;2(7736):1225–1227. doi: 10.1016/s0140-6736(71)90543-5. [DOI] [PubMed] [Google Scholar]
  3. Bayer M. E., Blumberg B. S., Werner B. Particles associated with Australia antigen in the sera of patients with leukaemia, Down's Syndrome and hepatitis. Nature. 1968 Jun 15;218(5146):1057–1059. doi: 10.1038/2181057a0. [DOI] [PubMed] [Google Scholar]
  4. Dane D. S., Cameron C. H., Briggs M. Virus-like particles in serum of patients with Australia-antigen-associated hepatitis. Lancet. 1970 Apr 4;1(7649):695–698. doi: 10.1016/s0140-6736(70)90926-8. [DOI] [PubMed] [Google Scholar]
  5. Hoofnagle J. H., Gerety R. J., Barker L. F. Antibody to hepatitis-B-virus core in man. Lancet. 1973 Oct 20;2(7834):869–873. doi: 10.1016/s0140-6736(73)92004-7. [DOI] [PubMed] [Google Scholar]
  6. Kaplan P. M., Greenman R. L., Gerin J. L., Purcell R. H., Robinson W. S. DNA polymerase associated with human hepatitis B antigen. J Virol. 1973 Nov;12(5):995–1005. doi: 10.1128/jvi.12.5.995-1005.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Plow E. F., Hougie C., Edgington T. S. Neoantigenic expressions engendered by plasmin cleavage of fibrinogen. J Immunol. 1971 Nov;107(5):1496–1500. [PubMed] [Google Scholar]
  8. Robinson W. S., Robinson H. L. DNA polymerase in defective Rous sarcoma virus. Virology. 1971 May;44(2):457–462. doi: 10.1016/0042-6822(71)90278-9. [DOI] [PubMed] [Google Scholar]
  9. Vyas G. N., Shulman N. R. Hemagglutination assay for antigen and antibody associated with viral hepatitis. Science. 1970 Oct 16;170(3955):332–333. doi: 10.1126/science.170.3955.332. [DOI] [PubMed] [Google Scholar]

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