Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1974 Jun;13(6):1254–1262. doi: 10.1128/jvi.13.6.1254-1262.1974

Base Composition and Hybridization Studies of the Three Double-Stranded RNA Segments of Bacteriophage φ6 1

J L Van Etten 1,2, A K Vidaver 1,2, R K Koski 1,2, J P Burnett 1,2
PMCID: PMC355445  PMID: 4833610

Abstract

The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage φ6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of the large dsRNA segment. The two smaller RNA segments reanneal rapidly even at low temperatures; in contrast, the large dsRNA reannealed only at higher temperatures. Evidence is also presented which suggests that the dsRNAs may contain a short single-stranded RNA tail.

Full text

PDF
1254

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  2. Brakke M. K., Van Pelt N. Linear-log sucrose gradients for estimating sedimentation coefficients of plant viruses and nucleic acids. Anal Biochem. 1970 Nov;38(1):56–64. doi: 10.1016/0003-2697(70)90155-7. [DOI] [PubMed] [Google Scholar]
  3. Brakke M. K., Van Pelt N. Properties of infectious ribonucleic acid from wheat streak mosaic virus. Virology. 1970 Nov;42(3):699–706. doi: 10.1016/0042-6822(70)90315-6. [DOI] [PubMed] [Google Scholar]
  4. Diener T. O., Schneider I. R. Virus degradation and nucleic acid release in single-phase phenol systems. Arch Biochem Biophys. 1968 Mar 20;124(1):401–412. doi: 10.1016/0003-9861(68)90344-5. [DOI] [PubMed] [Google Scholar]
  5. Franklin R. M. Replication of bacteriophage ribonucleic acid: some physical properties of single-stranded, double-stranded, and branched viral ribonucleic acid. J Virol. 1967 Feb;1(1):64–75. doi: 10.1128/jvi.1.1.64-75.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Katz L., Penman S. The solvent denaturation of double-stranded RNA from poliovirus infected HeLa cells. Biochem Biophys Res Commun. 1966 May 25;23(4):557–560. doi: 10.1016/0006-291x(66)90765-0. [DOI] [PubMed] [Google Scholar]
  7. Semancik J. S., Vidaver A. K., Van Etten J. L. Characterization of segmented double-helical RNA from bacteriophage phi6. J Mol Biol. 1973 Aug 25;78(4):617–625. doi: 10.1016/0022-2836(73)90283-0. [DOI] [PubMed] [Google Scholar]
  8. Van Etten J. L., Vidaver A. K., Koski R. K., Semancik J. S. RNA polymerase activity associated with bacteriophage phi 6. J Virol. 1973 Sep;12(3):464–471. doi: 10.1128/jvi.12.3.464-471.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Vidaver A. K., Koski R. K., Van Etten J. L. Bacteriophage phi6: a Lipid-Containing Virus of Pseudomonas phaseolicola. J Virol. 1973 May;11(5):799–805. doi: 10.1128/jvi.11.5.799-805.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wood H. A. Viruses with double-stranded RNA genomes. J Gen Virol. 1973 Jun;20(Suppl):61–85. doi: 10.1099/0022-1317-20-Supplement-61. [DOI] [PubMed] [Google Scholar]
  11. Zaitlin M., Hariharasubramanian V. An improvement in a procedure for counting tritium and carbon-14 in polyacrylamide gels. Anal Biochem. 1970 May;35(1):296–297. doi: 10.1016/0003-2697(70)90038-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES