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. 1974 Jul;14(1):1–7. doi: 10.1128/jvi.14.1.1-7.1974

Serological Characterization of the Three Major Proteins of Vesicular Stomatitis Virus

Bernhard Dietzschold 1, Lothar G Schneider 1, James H Cox 1
PMCID: PMC355470  PMID: 4365717

Abstract

The three major proteins of vesicular stomatitis virus-Indiana, glycoprotein (G), nucleoprotein (N), and membrane protein (M), were isolated and characterized by means of specific monocomponent antisera. G, N, and M proteins are distinct, nonrelated antigens with specific serological properties. The G protein is the only antigen inducing the formation of virus-neutralizing antibodies and was shown to confer immunity to mice. Specific complement-fixing and precipitating activity was demonstrated for each of the three antisera. The future use of isolated rhabdovirus components and of monospecific antisera is considered for therapeutic and diagnostic purposes as well as for virus strain differentiation and classification work.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arko R. J., Wiktor T. J., Sikes R. K. Laboratory techniques in rabies: the antibody binding test for vaccine potency. Monogr Ser World Health Organ. 1973;(23):292–294. [PubMed] [Google Scholar]
  2. Bishop D. H., Roy P. Dissociation of vesicular stomatitis virus and relation of the virion proteins to the viral transcriptase. J Virol. 1972 Aug;10(2):234–243. doi: 10.1128/jvi.10.2.234-243.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bolognesi D. P., Bauer H. Polypeptides of avian RNA tumor viruses. 1. Isolation and physical and chemical analysis. Virology. 1970 Dec;42(4):1097–1112. doi: 10.1016/0042-6822(70)90357-0. [DOI] [PubMed] [Google Scholar]
  4. Friesen A. D., Jamieson J. C., Ashton F. E. Effect of nonionic detergent on fractionation of proteins by isoelectric focusing. Anal Biochem. 1971 May;41(1):149–157. doi: 10.1016/0003-2697(71)90200-4. [DOI] [PubMed] [Google Scholar]
  5. Kelley J. M., Emerson S. U., Wagner R. R. The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to and reacts with neutralizing antibody. J Virol. 1972 Dec;10(6):1231–1235. doi: 10.1128/jvi.10.6.1231-1235.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. McSharry J., Benzinger R. Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation". Virology. 1970 Mar;40(3):745–746. doi: 10.1016/0042-6822(70)90219-9. [DOI] [PubMed] [Google Scholar]
  8. Mudd J. A., Summers D. F. Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Oct;42(2):328–340. doi: 10.1016/0042-6822(70)90277-1. [DOI] [PubMed] [Google Scholar]
  9. Oshiro Y., Eylar E. H. Physical and chemical studies on glycoproteins. IV. The influence of sialic acid on the conformation of fetuin. Arch Biochem Biophys. 1969 Mar;130(1):227–234. doi: 10.1016/0003-9861(69)90028-9. [DOI] [PubMed] [Google Scholar]
  10. SPIRO R. G. THE CARBOHYDRATE UNITS OF THYROGLOBULIN. J Biol Chem. 1965 Apr;240:1603–1610. [PubMed] [Google Scholar]
  11. STOKER M., MACPHERSON I. SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES. Nature. 1964 Sep 26;203:1355–1357. doi: 10.1038/2031355a0. [DOI] [PubMed] [Google Scholar]
  12. Schneider L. G., Dietzschold B., Dierks R. E., Matthaeus W., Enzmann P. J., Strohmaier K. Rabies group-specific ribonucleoprotein antigen and a test system for grouping and typing of rhabdoviruses. J Virol. 1973 May;11(5):748–755. doi: 10.1128/jvi.11.5.748-755.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schneider L. G., Horzinek M., Matheka H. D. Purification of rabies virus from tissue culture. Arch Gesamte Virusforsch. 1971;34(4):351–359. doi: 10.1007/BF01242982. [DOI] [PubMed] [Google Scholar]
  14. Sedwick W. D., Wiktor T. J. Reproducible plaquing system for rabies, lymphocytic choriomeningitis,k and other ribonucleic acid viruses in BHK-21-13S agarose suspensions. J Virol. 1967 Dec;1(6):1224–1226. doi: 10.1128/jvi.1.6.1224-1226.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Seligmann E. B., Jr Laboratory techniques in rabies: the NIH test for potency. Monogr Ser World Health Organ. 1973;(23):279–286. [PubMed] [Google Scholar]
  16. Wagner R. R., Schnaitman T. C., Snyder R. M., Schnaitman C. A. Protein composition of the structural components of vesicular stomatitis virus. J Virol. 1969 Jun;3(6):611–618. doi: 10.1128/jvi.3.6.611-618.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wiktor T. J. Laboratoty techniques in rabies: tissue culture methods. Monogr Ser World Health Organ. 1973;(23):101–123. [PubMed] [Google Scholar]

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