Abstract
Temperature-sensitive mutants of simian virus 40 (SV40) have been classified as those that are blocked prior to viral DNA synthesis at the restrictive temperature, “early” mutants, and those harboring a defect later in the replication cycle, “late” mutants. Mutants of the A and D complementation groups are early, those of the B, C, and BC groups are late. Our results confirm earlier reports that A mutants are defective in a function required for the initiation of each round of viral DNA synthesis. D mutants, on the other hand, continue viral DNA replication at the restrictive temperature after preincubation at the permissive temperature. The length of time required for D function to be expressed at the permissive temperature—after which infection proceeds unabated on shifting of the cultures to the restrictive temperature—is 10 to 20 h. The viral DNA synthesized in D mutants under these conditions progresses in normal fashion through replicative intermediate molecules to mature component I and II DNA molecules.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chou J. Y., Martin R. G. Complementation analysis of simian virus 40 mutants. J Virol. 1974 May;13(5):1101–1109. doi: 10.1128/jvi.13.5.1101-1109.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dubbs D. R., Rachmeler M., Kit S. Recombination between temperature-sensitive mutants of simian virus 40. Virology. 1974 Jan;57(1):161–174. doi: 10.1016/0042-6822(74)90117-2. [DOI] [PubMed] [Google Scholar]
- Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
- Kimura G., Dulbecco R. A temperature-sensitive mutant of simian virus 40 affecting transforming ability. Virology. 1973 Apr;52(2):529–534. doi: 10.1016/0042-6822(73)90348-6. [DOI] [PubMed] [Google Scholar]
- Kimura G., Dulbecco R. Isolation and characterization of temperature-sensitive mutants of simian virus 40. Virology. 1972 Aug;49(2):394–403. doi: 10.1016/0042-6822(72)90492-8. [DOI] [PubMed] [Google Scholar]
- Kimura G. Genetic evidence for SV40 gene function in enhancement of replication of human adenovirus in simian cells. Nature. 1974 Apr 12;248(449):590–592. doi: 10.1038/248590a0. [DOI] [PubMed] [Google Scholar]
- Robb J. A., Martin R. G. Genetic analysis of simian virus 40. 3. Characterization of a temperature-sensitive mutant blocked at an early stage of productive infection in monkey cells. J Virol. 1972 Jun;9(6):956–968. doi: 10.1128/jvi.9.6.956-968.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robb J. A., Martin R. G. Genetic analysis of simian virus 40. I. Description of microtitration and replica-plating techniques for virus. Virology. 1970 Aug;41(4):751–760. doi: 10.1016/0042-6822(70)90439-3. [DOI] [PubMed] [Google Scholar]
- Tegtmeyer P., Dohan C., Jr, Reznikoff C. Inactivating and mutagenic effects of nitrosoguanidine on simian virus 40. Proc Natl Acad Sci U S A. 1970 Jul;66(3):745–752. doi: 10.1073/pnas.66.3.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tegtmeyer P., Ozer H. L. Temperature-sensitive mutants of simian virus 40: infection of permissive cells. J Virol. 1971 Oct;8(4):516–524. doi: 10.1128/jvi.8.4.516-524.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tegtmeyer P. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J Virol. 1972 Oct;10(4):591–598. doi: 10.1128/jvi.10.4.591-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]