Role of the iron axial ligands of heme carrier HasA in heme uptake and release.

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An additional sentence should be inserted in the right column at the end of the “Discussion.” “In addition, Yukl et al. (49) and Jepkorir et al. (50) propose a model in HasAp from Pseudomonas aeruginosa in which heme is initially bound by the Tyr⁷⁵ to form a high-spin heme-protein complex before coordination of the His³² ligand upon closing of the His loop over the heme.” These additions do not result in any change in the conclusions of the article.

• 49. Yukl, E. T., Jepkorir, G., Alontaga, A. Y., Pautsch, L., Rodríguez, J. C., Rivera, M., and Moënne-Loccoz, P. (2010) Kinetic and spectroscopic studies of hemin acquisition in the hemophore HasAp from Pseudomonas aeruginosa. Biochemistry 49, 6646–6654

• 50. Jepkorir, G., Rodríguez, J. C., Rui, H., Im, W., Lovell, S., Battaile, K. P., Alontaga, A. Y., Yukl, E. T., Moënne-Loccoz, P., and Rivera, M. (2010) Structural, NMR spectroscopic, and computational investigation of hemin loading in the hemophore HasAp from Pseudomonas aeruginosa. J. Am. Chem. Soc. 132, 9857–9872