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. 1974 Aug;14(2):191–197. doi: 10.1128/jvi.14.2.191-197.1974

Analysis of the In Vitro Product of an RNA-Dependent RNA Polymerase Isolated from Influenza Virus-Infected Cells

Lawrence A Caliguiri 1,1, Richard W Compans 1
PMCID: PMC355502  PMID: 4858784

Abstract

The products synthesized in vitro by an RNA-dependent RNA polymerase isolated from influenza virus-infected BHK21-F cells were analyzed by velocity sedimentation, annealing techniques, and acrylamide-agarose gel electrophoresis. Approximately 50% of the RNA synthesized in vitro remains associated with the 50 to 70S ribonucleoprotein complex containing polymerase activity; the remainder of the RNA polymerase product sediments heterogeneously with a peak at 13S. At least 90% of the in vitro product hybridizes with virion RNA. If polypeptides are labeled early in the growth cycle, both the P and NP polypeptides are detected in the ribonucleoprotein complex by acrylamide gel electrophoresis. The results suggest that the polypeptide composition and the products of the cell-associated RNA polymerase are similar to those of the RNA transcriptase associated with influenza virus particles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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