Abstract
We have studied the properties of presumptive point mutants in the D2a region of bacteriophage T4. Dominance tests showed that the D2a mutation was recessive to the wild-type allele. The mutations were shown to map in the D2a region by complementation against rII deletions. The D2a mutations were also located between gene 52 and rIIB by two- and three-factor crosses. The mutants are located at at least two distinct loci in the D2a region. The point mutants grow normally on all hosts tested and none of the mutants makes T4 endonuclease IV. We propose the name “denB” for the D2a locus.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bautz F. A., Bautz E. K. Mapping of deletions in a non-essential region of the phage T4 genome. J Mol Biol. 1967 Sep 14;28(2):345–355. doi: 10.1016/s0022-2836(67)80014-7. [DOI] [PubMed] [Google Scholar]
- Benzer S. ON THE TOPOGRAPHY OF THE GENETIC FINE STRUCTURE. Proc Natl Acad Sci U S A. 1961 Mar;47(3):403–415. doi: 10.1073/pnas.47.3.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berger H., Kozinski A. W. Suppression of T4D ligase mutations by rIIa and rIIb mutations. Proc Natl Acad Sci U S A. 1969 Nov;64(3):897–904. doi: 10.1073/pnas.64.3.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bruner R., Solomon D., Berger T. Presumptive D2a point mutants of bacteriophage T4. J Virol. 1973 Oct;12(4):946–947. doi: 10.1128/jvi.12.4.946-947.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bruner R., Souther A., Suggs S. Stability of cytosine-containing deoxyribonucleic acid after infection by certain T4 rII-D deletion mutants. J Virol. 1972 Jul;10(1):88–92. doi: 10.1128/jvi.10.1.88-92.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chase M, Doermann A H. High Negative Interference over Short Segments of the Genetic Structure of Bacteriophage T4. Genetics. 1958 May;43(3):332–353. doi: 10.1093/genetics/43.3.332. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Depew R. E., Cozzarelli N. R. Genetics and physiology of bacteriophage T4 3'-phosphatase: evidence for involvement of the enzyme in T4 DNA metabolism. J Virol. 1974 Apr;13(4):888–897. doi: 10.1128/jvi.13.4.888-897.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dove W. The extent of rII deletions in phage T4. Genet Res. 1968 Apr;11(2):215–219. doi: 10.1017/s001667230001140x. [DOI] [PubMed] [Google Scholar]
- Hercules K., Munro J. L., Mendelsohn S., Wiberg J. S. Mutants in a nonessential gene of bacteriophage T4 which are defective in the degradation of Escherichia coli deoxyribonucleic acid. J Virol. 1971 Jan;7(1):95–105. doi: 10.1128/jvi.7.1.95-105.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karam J. D., Barker B. Properties of bacteriophage T4 mutants defective in gene 30 (deoxyribonucleic acid ligase) and the rII gene. J Virol. 1971 Feb;7(2):260–266. doi: 10.1128/jvi.7.2.260-266.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karam J. D. DNA replication of phage T4 rII mutants without polynucleotide ligase (gene 30). Biochem Biophys Res Commun. 1969 Oct 22;37(3):416–422. doi: 10.1016/0006-291x(69)90931-0. [DOI] [PubMed] [Google Scholar]
- Kasai T., Bautz E. K. Regulation of gene-specific RNA synthesis in bacteriophage T4. J Mol Biol. 1969 May 14;41(3):401–417. doi: 10.1016/0022-2836(69)90284-8. [DOI] [PubMed] [Google Scholar]
- King J., Laemmli U. K. Bacteriophage T4 tail assembly: structural proteins and their genetic identification. J Mol Biol. 1973 Apr 5;75(2):315–337. doi: 10.1016/0022-2836(73)90024-7. [DOI] [PubMed] [Google Scholar]
- Krisch H. M., Shah D. B., Berger H. Replication and recombination in ligase-deficient rII bacteriophage T4D. J Virol. 1971 Apr;7(4):491–498. doi: 10.1128/jvi.7.4.491-498.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kutter E. M., Wiberg J. S. Degradation of cytosin-containing bacterial and bacteriophage DNA after infection of Escherichia coli B with bacteriophage T4D wild type and with mutants defective in genes 46, 47 and 56. J Mol Biol. 1968 Dec;38(3):395–411. doi: 10.1016/0022-2836(68)90394-x. [DOI] [PubMed] [Google Scholar]
- Sadowski P. D., Hurwitz J. Enzymatic breakage of deoxyribonucleic acid. I. Purification and properties of endonuclease II from T4 phage-infected Escherichia coli. J Biol Chem. 1969 Nov 25;244(22):6182–6191. [PubMed] [Google Scholar]
- Sadowski P. D., Hurwitz J. Enzymatic breakage of deoxyribonucleic acid. II. Purification and properties of endonuclease IV from T4 phage-infected Escherichia coli. J Biol Chem. 1969 Nov 25;244(22):6192–6198. [PubMed] [Google Scholar]
- Sadowski P. D., Kerr C. Degradation of Escherichia coli B deoxyribonucleic acid after infection with deoxyribonucleic acid-defective amber mutants of bacteriophage T7. J Virol. 1970 Aug;6(2):149–155. doi: 10.1128/jvi.6.2.149-155.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sadowski P. D., Vetter D. Control of T4 endonuclease IV by the D2a region of bacteriophage T4. Virology. 1973 Aug;54(2):544–546. doi: 10.1016/0042-6822(73)90165-7. [DOI] [PubMed] [Google Scholar]
- Sederoff R., Bolle A., Epstein R. H. A method for the detection of specific T4 messenger RNAs by hybridization competition. Virology. 1971 Aug;45(2):440–455. doi: 10.1016/0042-6822(71)90344-8. [DOI] [PubMed] [Google Scholar]
- Snustad D. P., Warner H. R., Parson K. A., Anderson D. L. Nuclear disruption after infection of Escherichia coli with a bacteriophage T4 mutant unable to induce endonuclease II. J Virol. 1972 Jul;10(1):124–133. doi: 10.1128/jvi.10.1.124-133.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Souther A., Bruner R., Elliott J. Degradation of Escherichia coli chromosome after infection by bacteriophage T4: role of bacteriophage gene D2a. J Virol. 1972 Nov;10(5):979–984. doi: 10.1128/jvi.10.5.979-984.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TESSMAN I. The induction of large deletions by nitrous acid. J Mol Biol. 1962 Oct;5:442–445. doi: 10.1016/s0022-2836(62)80033-3. [DOI] [PubMed] [Google Scholar]
