Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1974 Sep;14(3):441–450. doi: 10.1128/jvi.14.3.441-450.1974

Transcribing Complexes in Cells Infected by Vesicular Stomatitis Virus and Rabies Virus

Louis Perez Villarreal 1, John J Holland 1
PMCID: PMC355536  PMID: 4369391

Abstract

We analyzed cell extracts from BHK21 cells infected with vesicular stomatitis virus (VSV) and rabies virus for in vitro RNA polymerase activity. Cells infected with VSV B virions exhibited several complexes with in vitro RNA polymerase activity in sucrose gradients. These complexes synthesize VSV transcriptase product (4 to 18S) polyadenylated in RNA complementary to virion RNA. Cells infected with a high multiplicity of B virions and T particles show only one RNA polymerase complex active in vitro. This complex sediments at 110S and makes only small (2S) RNA. Carrier BHK21 cells persistently noncytopathically infected with VSV contain several complexes active in RNA polymerase, but both exhibit very low activity. Cytoplasms of cells noncytopathically infected with rabies virus also show very low levels of a complex containing RNA polymerase activity. No transcriptase nor any other in vitro polymerase activity could be found associated with purified rabies virions, although they do carry out primary transcription in cells treated with actinomycin D and cycloheximide before infection.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaslestad H. G., Clark H. F., Bishop D. H., Koprowski H. Comparison of the ribonucleic acid polymerases of two rhabdoviruses, Kern Canyon virus and vesicular stomatitis virus. J Virol. 1971 Jun;7(6):726–735. doi: 10.1128/jvi.7.6.726-735.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BURDON R. H., SMELLIE R. M. The incorporation of uridine 5'-triphosphate into ribonucleic acid by enzyme fractions from Ehrlich ascites carcinoma cells. Biochim Biophys Acta. 1961 Jul 22;51:153–162. doi: 10.1016/0006-3002(61)91026-5. [DOI] [PubMed] [Google Scholar]
  3. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Banerjee A. K., Rhodes D. P. In vitro synthesis of RNA that contains polyadenylate by virion-associated RNA polymerase of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3566–3570. doi: 10.1073/pnas.70.12.3566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doyle M., Holland J. J. Prophylaxis and immunization in mice by use of virus-free defective T particles to protect against intracerebral infection by vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2105–2108. doi: 10.1073/pnas.70.7.2105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ehrenfeld E., Summers D. F. Adenylate-rich sequences in vesicular stomatitis virus messenger ribonucleic acid. J Virol. 1972 Oct;10(4):683–688. doi: 10.1128/jvi.10.4.683-688.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Galet H., Prevec L. Polyadenylate synthesis by extracts from L cells infected with vesicular stomatitis virus. Nat New Biol. 1973 Jun 13;243(128):200–203. doi: 10.1038/newbio243200a0. [DOI] [PubMed] [Google Scholar]
  8. Galet H., Shedlarski J. G., Jr, Prevec L. Ribonucleic acid polymerase induced in L-cells infected with vesicular stomatitis virus. Can J Biochem. 1973 May;51(5):721–729. doi: 10.1139/o73-089. [DOI] [PubMed] [Google Scholar]
  9. Huang A. S., Baltimore D., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus. 3. Multiple complementary messenger RNA molecules. Virology. 1970 Dec;42(4):946–957. doi: 10.1016/0042-6822(70)90343-0. [DOI] [PubMed] [Google Scholar]
  10. Knudson D. L. Rhabdoviruses. J Gen Virol. 1973 Jun;20(Suppl):105–130. doi: 10.1099/0022-1317-20-Supplement-105. [DOI] [PubMed] [Google Scholar]
  11. Mudd J. A., Summers D. F. Polysomal ribonucleic acid of vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Dec;42(4):958–968. doi: 10.1016/0042-6822(70)90344-2. [DOI] [PubMed] [Google Scholar]
  12. Perlman S. M., Huang A. S. RNA synthesis of vesicular stomatitis virus. V. Interactions between transcription and replication. J Virol. 1973 Dec;12(6):1395–1400. doi: 10.1128/jvi.12.6.1395-1400.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Perrault J., Holland J. J. Absence of transcriptase activity or transcription-inhibiting ability in defective interfering particles of vesicular stomatitis virus. Virology. 1972 Oct;50(1):150–170. [PubMed] [Google Scholar]
  14. Randles J. W., Francki R. I. Infectious nucleocapsid particles of lettuce necrotic yellows virus with RNA-dependent RNA polymerase activity. Virology. 1972 Oct;50(1):297–300. doi: 10.1016/0042-6822(72)90377-7. [DOI] [PubMed] [Google Scholar]
  15. Reichmann M. E., Villarreal L. P., Kohne D., Lesnaw J., Holland J. J. RNA polymerase activity and poly(A) synthesizing activity in defective T particles of vesicular stomatitis virus. Virology. 1974 Mar;58(1):240–249. doi: 10.1016/0042-6822(74)90158-5. [DOI] [PubMed] [Google Scholar]
  16. Sokol F., Clark H. F. Phosphoproteins, structural components of rhabdoviruses. Virology. 1973 Mar;52(1):246–263. doi: 10.1016/0042-6822(73)90413-3. [DOI] [PubMed] [Google Scholar]
  17. Sokol F., Kuwert E., Wiktor T. J., Hummeler K., Koprowski H. Purification of rabies virus grown in tissue culture. J Virol. 1968 Aug;2(8):836–849. doi: 10.1128/jvi.2.8.836-849.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Soria M., Huang A. S. Association of polyadenylic acid with messenger RNA of vesicular stomatitis virus. J Mol Biol. 1973 Jul 5;77(3):449–455. doi: 10.1016/0022-2836(73)90450-6. [DOI] [PubMed] [Google Scholar]
  19. Stampfer M., Baltimore D., Huang A. S. Absence of interference during high-multiplicity infection by clonally purified vesicular stomatitis virus. J Virol. 1971 Mar;7(3):409–411. doi: 10.1128/jvi.7.3.409-411.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Villarreal L. P., Holland J. J. Synthesis of poly(A) in vitro by purified virions of vesicular stomatitis virus. Nat New Biol. 1973 Nov 7;246(149):17–19. doi: 10.1038/newbio246017a0. [DOI] [PubMed] [Google Scholar]
  21. WAGNER R. R., LEVEY A. H., SNYDER R. M., RATCLIFF G. A., Jr, HYATT D. F. BIOLOGIC PROPERTIES OF TWO PLAQUE VARIANTS OF VESICULAR STOMATITIS VIRUS (INDIANA SEROTYPE). J Immunol. 1963 Jul;91:112–122. [PubMed] [Google Scholar]
  22. Wagner R. R., Schnaitman T. C., Snyder R. M., Schnaitman C. A. Protein composition of the structural components of vesicular stomatitis virus. J Virol. 1969 Jun;3(6):611–618. doi: 10.1128/jvi.3.6.611-618.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wilkie N. M., Smellie R. M. Chain extension of ribonucleic acid by enzymes from rat liver cytoplasm. Biochem J. 1968 Oct;109(4):485–494. doi: 10.1042/bj1090485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wilkie N. M., Smellie R. M. Polyribonucleotide synthesis by subfractions of microsomes from rat liver. Biochem J. 1968 Sep;109(2):229–238. doi: 10.1042/bj1090229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wykes J. R., Smellie R. M. The synthesis of polyribonucleotides by cytoplasmic enzymes. Biochem J. 1966 May;99(2):347–355. doi: 10.1042/bj0990347. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES