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. 1974 Sep;14(3):479–484. doi: 10.1128/jvi.14.3.479-484.1974

Phospholipase Activity in Bacteriophage-Infected Escherichia coli I. Demonstration of a T4 Bacteriophage-Associated phospholipase

E T Nelson 1, C S Buller 1
PMCID: PMC355540  PMID: 4604905

Abstract

Phospholipase activity has been found to be associated with T4 phage and T4 ghost particles. The attachment of the phospholipase to the phage persists during purification through cesium chloride gradients and dialysis, indicating that it is firmly bound. The presence of the enzymatic activity on T4 ghosts suggests that it is not normally packaged within the head of the virus. The enzyme has specificity for phosphatidylglycerol and its activity is stimulated by 0.1% Triton X-100 and 20% methanol. It does not have a requirement for Ca2+ and is inactivated at temperatures above 60 C. The association of the phospholipase with T4 phage grown in a phospholipase-deficient host and its absence on unsuppressed T4amtA3 suggests that it may be phage gene specific.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett J., Glavinovich J., Liskay R., Wulff D. L., Cronan J. E., Jr Phospholipid hydrolysis in Escherichia coli infected with rapid lysis mutants of Phage T4. Virology. 1971 Feb;43(2):516–518. doi: 10.1016/0042-6822(71)90326-6. [DOI] [PubMed] [Google Scholar]
  2. Bode W. Lysis inhibition in Escherichia coli infected with bacteriophage T4. J Virol. 1967 Oct;1(5):948–955. doi: 10.1128/jvi.1.5.948-955.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradley W. E., Astrachan L. Occurrence of lysophosphatides in bacteriophage T4rII-infected Escherichia coli S-6. J Virol. 1971 Oct;8(4):437–445. doi: 10.1128/jvi.8.4.437-445.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cavard D., Rampini C., Barbu E., Polonovski J. Activité phospholipasique et autres modifications du métabolisme des phospholipides consécutives a l'action des colicines sur E. coli. Bull Soc Chim Biol (Paris) 1968 Dec;50(9):1455–1471. [PubMed] [Google Scholar]
  5. Cronan J. E., Jr, Wulff D. L. A role for phospholipid hydrolysis in the lysis of Escherichia coli infected with bacteriophage T4. Virology. 1969 Jun;38(2):241–246. doi: 10.1016/0042-6822(69)90365-1. [DOI] [PubMed] [Google Scholar]
  6. Doi O., Oki M., Nojima S. Two kinds of phospholipase A and lysophospholipase in Escherichia coli. Biochim Biophys Acta. 1972 Feb 21;260(2):244–258. [PubMed] [Google Scholar]
  7. Duckworth D. H. The metabolism of T4 phage ghost-infected cells. I. Macromolecular synthesis and ransport of nucleic acid and protein precursors. Virology. 1970 Mar;40(3):673–684. doi: 10.1016/0042-6822(70)90212-6. [DOI] [PubMed] [Google Scholar]
  8. Josslin R. Physiological studies on the t gene defect in T4-infected Escherichia coli. Virology. 1971 Apr;44(1):101–107. doi: 10.1016/0042-6822(71)90157-7. [DOI] [PubMed] [Google Scholar]
  9. Josslin R. The effect of phage T4 infection on phospholipid hydrolysis in Escherichia coli. Virology. 1971 Apr;44(1):94–100. doi: 10.1016/0042-6822(71)90156-5. [DOI] [PubMed] [Google Scholar]
  10. Josslin R. The lysis mechanism of phage T4: mutants affecting lysis. Virology. 1970 Mar;40(3):719–726. doi: 10.1016/0042-6822(70)90216-3. [DOI] [PubMed] [Google Scholar]
  11. KATES M., ADAMS G. A., MARTIN S. M. LIPIDS OF SERRATIA MARCESCENS. Can J Biochem. 1964 Apr;42:461–479. doi: 10.1139/o64-054. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Mukai F., Streisinger G., Miller B. The mechanism of lysis in phage T4-infected cells. Virology. 1967 Nov;33(3):398–404. doi: 10.1016/0042-6822(67)90115-8. [DOI] [PubMed] [Google Scholar]
  14. PUCK T. T., LEE H. H. Mechanism of cell wall penetration by viruses. I. An increase in host cell permeability induced by bacteriophage infection. J Exp Med. 1954 May 1;99(5):481–494. doi: 10.1084/jem.99.5.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PUCK T. T., LEE H. H. Mechanism of cell wall penetration by viruses. II. Demonstration of cyclic permeability change accompanying virus infection of Escherichia coli B cells. J Exp Med. 1955 Feb 1;101(2):151–175. doi: 10.1084/jem.101.2.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Peterson R. H., Buller C. S. Phospholipid metabolism in T4 bacteriophage infected Escherichia coli K-12 (lambda). J Virol. 1969 May;3(5):463–468. doi: 10.1128/jvi.3.5.463-468.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rampini C., Lux M., Cavard D., Barbu E. Activation par chauffage d'enzymes bactériens agissant sur les phospholipids. C R Acad Sci Hebd Seances Acad Sci D. 1969 Jan 6;268(1):206–209. [PubMed] [Google Scholar]
  18. STREISINGER G., MUKAI F., DREYER W. J., MILLER B., HORIUCHI S. Mutations affecting the lysozyme of phage T4. Cold Spring Harb Symp Quant Biol. 1961;26:25–30. doi: 10.1101/sqb.1961.026.01.007. [DOI] [PubMed] [Google Scholar]
  19. Scandella C. J., Kornberg A. A membrane-bound phospholipase A1 purified from Escherichia coli. Biochemistry. 1971 Nov 23;10(24):4447–4456. doi: 10.1021/bi00800a015. [DOI] [PubMed] [Google Scholar]
  20. Silver S., Levine E., Spielman P. M. Cation fluxes and permeability changes accompanying bacteriophage infection of Escherichia coli. J Virol. 1968 Aug;2(8):763–771. doi: 10.1128/jvi.2.8.763-771.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]

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