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. 1974 Sep;14(3):493–502. doi: 10.1128/jvi.14.3.493-502.1974

Incomplete and Aberrant Particles of Actinophage MSP2 from Lysates of Streptomyces venezuelae

Lily A Jones a, Marianne L Hahn a,1, Alton R Taylor a
PMCID: PMC355542  PMID: 4852395

Abstract

Lysates of actinophage MSP2, propagated on Streptomyces venezuelae S13, contain at least 1011 PFU/ml. During purification by centrifugation methods and by adsorption chromatography, a number of types of aberrant and incomplete phage particles were seen by electron microscopy. Infectious MSP2 had a buoyant density in CsCl of 1.52 g/cm3 and an absorbance at 260 nm relative to that at 280 nm (A260/A280) of 1.53. Empty capsids banded at 1.276 g/cm3 and partially filled capsids banded at 1.351 g/cm3, and A260/A280 ratios were 0.77 and 1.24, respectively. Two kinds of light capsids found in CsCl fractions of 1.278 g/cm3 probably include the 1.276 component. Some capsids were joined by tail-like structures. Ghosts and polyheads also were present. Aberrant particles observed by electron microscopy included two-tailed actinophage, phage with abnormal tail positions, and large-headed phage.

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Selected References

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