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. 2012 Nov 2;23(3):295–302. doi: 10.1093/glycob/cws152

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© The Author 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Fig. 4. — LARGE2dTM has polymerizing activity. (A) HPLC profile of the reaction products generated when LARGE2dTM was added to GlcA-β-MU in the presence of both donors, UDP-Xyl and UDP-GlcA, on the amide column GlycoSep N. Peaks labeled 1–3 were subjected to further purification in (B). S, unreacted substrate. Dashed line, %Buffer B. (B) Purification of GlcA-β-MU derivatives on an LC-18 column. Each peak isolated in (A) was purified and the main peak was collected (solid bar). (C) Q/TOF-MS analysis of the peaks isolated in (B). Assigned mass spectra in each peak confirmed that LARGE2dTM added repeating units of Xyl and GlcA onto the acceptor, GlcA-β-MU. dp, degree of polymerization.