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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Surgery. 2012 Aug;152(2):277–285. doi: 10.1016/j.surg.2012.05.006

Figure 5. siRNA treatments enhance inhibition of proliferation associated with 5-FU.

Figure 5

HCT116 cells were plated in 24 well plates at a density of 25,000 cells/well. Cells were transfected 12 h later with 100 nM NTC siRNA, 50 nM PIK3CA siRNA + 50 nM NTC siRNA, 50 nM KRAS siRNA + 50 nM NTC siRNA, or 50 nM PIK3CA siRNA + 50 nM KRAS siRNA. Media was exchanged 4 h later for either control media containing DMSO only, or media containing 1, 5, or 10 uM of 5-FU. Media was again exchanged for fresh drug media at 36 h post transfection. Proliferation was assessed by cell counting at 72 h (* p < 0.05 vs. control; † p < 0.05 vs. PIK3CA siRNA or KRAS siRNA alone).