Abstract
Radioactive labeling of proteins from guanidine-hydrochloride disrupted feline leukemia virus was done in vitro by attaching [3H]methyl groups to protein amino groups with reductive alkylation. When in vitro labeled disrupted virus was analyzed by gel filtration in the presence of 6 M guanidine-hydrochloride the expected six major and one minor protein peaks were detected. When the same preparation was analyzed by immunodiffusion, the three antigens tested were found to have retained their antigenic activity.
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- Fish W. W., Mann K. G., Tanford C. The estimation of polypeptide chain molecular weights by gel filtration in 6 M guanidine hydrochloride. J Biol Chem. 1969 Sep 25;244(18):4989–4994. [PubMed] [Google Scholar]
- Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses. J Virol. 1971 Nov;8(5):778–785. doi: 10.1128/jvi.8.5.778-785.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Graves D. C., Velicer L. F. Properties of feline leukemia virus. I. Chromatographic separation and analysis of the polypeptides. J Virol. 1974 Aug;14(2):349–365. doi: 10.1128/jvi.14.2.349-365.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nowinski R. C., Fleissner E., Sarkar N. H., Aoki T. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. II. Mammalian leukemia-sarcoma viruses. J Virol. 1972 Feb;9(2):359–366. doi: 10.1128/jvi.9.2.359-366.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rice R. H., Means G. E. Radioactive labeling of proteins in vitro. J Biol Chem. 1971 Feb 10;246(3):831–832. [PubMed] [Google Scholar]