Abstract
Polyoma virus was inactivated by treatment with β-propiolactone. T-antigen production, polyoma-RNA synthesis, induction of host DNA synthesis (measured by incorporation of labeled thymidine into the cell culture), and in vitro transforming ability were inactivated to a similar degree by various β-propiolactone concentrations (0.25% β-propiolactone reduced these functions approximately 96%), whereas plaque-forming ability and the ability of the virus to replicate its DNA and to synthesize capsid antigen were inactivated by a given concentration of β-propiolactone to a much greater degree (0.25% β-propiolactone led to a reduction of plaque-forming ability of over 8 logs). The significance of these data and their relationship to previously published experiments are discussed.
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- Basilico C., Di Mayorca G. Radiation target size of the lytic and the transforming ability of polyoma virus. Proc Natl Acad Sci U S A. 1965 Jul;54(1):125–127. doi: 10.1073/pnas.54.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benjamin T. L. Relative target sizes for the inactivation of the transforming and reproductive abilities of polyoma virus. Proc Natl Acad Sci U S A. 1965 Jul;54(1):121–124. doi: 10.1073/pnas.54.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Consigli R. A., Minocha H. C., Abo-Ahmed H. Multiplication of polyoma virus. II. Source of constituents for viral deoxyribonucleic acid and protein synthesis. J Bacteriol. 1966 Sep;92(3):789–791. doi: 10.1128/jb.92.3.789-791.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Consigli R. A., Zabielski J., Weil R. Plaque assay for polyoma virus on primary mouse kidney cell cultures. Appl Microbiol. 1973 Oct;26(4):627–628. doi: 10.1128/am.26.4.627-628.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EDDY B. E., ROWE W. P., HARTLEY J. W., STEWART S. E., HUEBNER R. J. Hemagglutination with the SE polyoma virus. Virology. 1958 Aug;6(1):290–291. doi: 10.1016/0042-6822(58)90078-3. [DOI] [PubMed] [Google Scholar]
- Herberman R. B., Ting R. C. Dissociation of multiple polyoma virus functions by ultraviolet irradiation. Proc Soc Exp Biol Med. 1969 Jun;131(2):461–464. doi: 10.3181/00379727-131-33902. [DOI] [PubMed] [Google Scholar]
- Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
- Koprowski H., Jensen F. C., Steplewski Z. Activation of production of infectious tumor virus SV40 in heterokaryon cultures. Proc Natl Acad Sci U S A. 1967 Jul;58(1):127–133. doi: 10.1073/pnas.58.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACPHERSON I., MONTAGNIER L. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS. Virology. 1964 Jun;23:291–294. doi: 10.1016/0042-6822(64)90301-0. [DOI] [PubMed] [Google Scholar]
- Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seemayer N. H., Defendi V. Analysis of minimal functions of simian virus 40. 3. Evidence for "host cell repair" of oncogenicity and infectivity of UV-irradiated simian virus 40. J Virol. 1974 Jan;13(1):36–41. doi: 10.1128/jvi.13.1.36-41.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seemayer N. H., Defendi V. Analysis of minimal functions of simian virus 40. II. Enhancement of oncogenic transformation in vitro by UV irradiation. J Virol. 1973 Dec;12(6):1265–1271. doi: 10.1128/jvi.12.6.1265-1271.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith G. L., Consigli R. A. Transient inhibition of polyoma virus synthesis by Sendai virus (parainfluenza I). I. Demonstration and nature of the inhibition by inactivated virus. J Virol. 1972 Dec;10(6):1091–1097. doi: 10.1128/jvi.10.6.1091-1097.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith G. L., Consigli R. A. Transient inhibition of polyoma virus synthesis by sendai virus (parainfluenza I). II. Mechanism of the interference by inactivated virus. J Virol. 1972 Dec;10(6):1098–1108. doi: 10.1128/jvi.10.6.1098-1108.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WINOCOUR E. Purification of polyoma virus. Virology. 1963 Feb;19:158–168. doi: 10.1016/0042-6822(63)90005-9. [DOI] [PubMed] [Google Scholar]
- Weil R., Kára J. Polyoma "tumor antigen": an activator of chromosome replication? Proc Natl Acad Sci U S A. 1970 Oct;67(2):1011–1017. doi: 10.1073/pnas.67.2.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]