Abstract
Polyoma virus was inactivated by treatment with β-propiolactone. T-antigen production, polyoma-RNA synthesis, induction of host DNA synthesis (measured by incorporation of labeled thymidine into the cell culture), and in vitro transforming ability were inactivated to a similar degree by various β-propiolactone concentrations (0.25% β-propiolactone reduced these functions approximately 96%), whereas plaque-forming ability and the ability of the virus to replicate its DNA and to synthesize capsid antigen were inactivated by a given concentration of β-propiolactone to a much greater degree (0.25% β-propiolactone led to a reduction of plaque-forming ability of over 8 logs). The significance of these data and their relationship to previously published experiments are discussed.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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