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. 1974 Oct;14(4):904–909. doi: 10.1128/jvi.14.4.904-909.1974

Studies on the Bacteriophage 2 Receptors of Pseudomonas aeruginosa

Francisco J Castillo 1, Pasquale F Bartell 1
PMCID: PMC355597  PMID: 4213969

Abstract

The lysogenization of Pseudomonas aeruginosa strain BI with phage 2 resulted in the loss of the capacity to adsorb the same phage. The absence of phage 2 receptors on the surface of the lysogenized strain BI(2)8 was confirmed by the failure of purified slime polysaccharide (SPB) or lipopolysaccharide (LPS) to inactivate phage 2. SPB and LPS from a phage 2-resistant strain also failed to inactivate phage 2 in contrast to the phage inactivation exhibited by the SPB and LPS obtained from the wild-type strain BI. Chemically, quantitative differences were apparent when the SPB and LPS of strains BI(2)8 and BI/2S2 were compared with those of the wild-type strain BI. The most striking difference noted was the absence of amino sugars in the SPB of strain BI/2S2. The SPB of strain BI(2)8 also contained a lower percentage of amino sugars compared with the SPB of the wild-type strain BI.

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Selected References

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  1. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  2. Bartell P. F., Orr T. E., Chudio B. Purification and Chemical Composition of the Protective Slime Antigen of Pseudomonas aeruginosa. Infect Immun. 1970 Nov;2(5):543–548. doi: 10.1128/iai.2.5.543-548.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartell P. F., Orr T. E., Lam G. K. Polysaccharide depolymerase associated with bacteriophage infection. J Bacteriol. 1966 Jul;92(1):56–62. doi: 10.1128/jb.92.1.56-62.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bartell P. F., Orr T. E., Reese J. F., Imaeda T. Interaction of Pseudomonas bacteriophage 2 with the slime polysaccharide and lipopolysaccharide of Pseudomonas aeruginosa strain B1. J Virol. 1971 Sep;8(3):311–317. doi: 10.1128/jvi.8.3.311-317.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DISCHE Z. Qualitative and quantitative colorimetric determination of heptoses. J Biol Chem. 1953 Oct;204(2):983–997. [PubMed] [Google Scholar]
  6. GEOBEL W. F., JESAITIS M. A. The somatic antigen of a phage-resistant variant of Phase II Shigella sonnei. J Exp Med. 1952 Nov;96(5):425–438. doi: 10.1084/jem.96.5.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HOLLOWAY B. W., COOPER G. N. Lysogenic conversion in Pseudomonas aeruginosa. J Bacteriol. 1962 Dec;84:1321–1324. doi: 10.1128/jb.84.6.1321-1324.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ikeda K., Egami F. Receptor substance for pyocin R. I. Partial purification and chemical properties. J Biochem. 1969 Apr;65(4):603–609. doi: 10.1093/oxfordjournals.jbchem.a129053. [DOI] [PubMed] [Google Scholar]
  9. Lee Y. C., Scocca J. R., Muir L. Rapid automatic analysis of sugar components in glycoproteins. I. Hexosamines. Anal Biochem. 1969 Mar;27(3):559–566. doi: 10.1016/0003-2697(69)90070-0. [DOI] [PubMed] [Google Scholar]
  10. Lindberg A. A. Bacteriophage receptors. Annu Rev Microbiol. 1973;27:205–241. doi: 10.1146/annurev.mi.27.100173.001225. [DOI] [PubMed] [Google Scholar]
  11. Lindberg A. A., Holme T. Influence of O side chains on the attachment of the Felix O-1 bacteriophage to Salmonella bacteria. J Bacteriol. 1969 Aug;99(2):513–519. doi: 10.1128/jb.99.2.513-519.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lindberg A. A. Studies of a receptor for felix O-1 phage in Salmonella minnesota. J Gen Microbiol. 1967 Aug;48(2):225–233. doi: 10.1099/00221287-48-2-225. [DOI] [PubMed] [Google Scholar]
  13. Liu P. V. Changes in somatic antigens of Pseudomonas aeruginosa induced by bacteriophages. J Infect Dis. 1969 Mar;119(3):237–246. doi: 10.1093/infdis/119.3.237. [DOI] [PubMed] [Google Scholar]
  14. Lüderitz O., Galanos C., Risse H. J., Ruschmann E., Schlecht S., Schmidt G., Schulte-Holthausen H., Wheat R., Westphal O., Schlosshardt J. Structural relationship of Salmonella O and R antigens. Ann N Y Acad Sci. 1966 Jun 30;133(2):349–374. doi: 10.1111/j.1749-6632.1966.tb52376.x. [DOI] [PubMed] [Google Scholar]
  15. OSBORN M. J. STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963 Sep;50:499–506. doi: 10.1073/pnas.50.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Partridge S. M. Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall. Biochem J. 1948;42(2):238–250. doi: 10.1042/bj0420238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reese J. F., Dimitracopoulos G., Bartell P. F. Factors influencing the adsorption of bacteriophage 2 to cells of Pseudomonas aeruginosa. J Virol. 1974 Jan;13(1):22–27. doi: 10.1128/jvi.13.1.22-27.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rosen S. M., Zeleznick L. D., Fraenkel D., Wiener I. M., Osborn M. J., Horecker B. L. Characterization of the cell wall lipopolysaccharide of a mutant of Salmonella typhimurium lacking phosphomannose isomerase. Biochem Z. 1965 Aug 19;342(4):375–386. [PubMed] [Google Scholar]
  19. Sensakovic J. W., Bartell P. F. The slime of Pseudomonas aeruginosa: biological characterization and possible role in experimental infection. J Infect Dis. 1974 Feb;129(2):101–109. doi: 10.1093/infdis/129.2.101. [DOI] [PubMed] [Google Scholar]
  20. Smit J. A., Hugo N., de Klerk H. C. A receptor for a Proteus vulgaris bacteriocin. J Gen Virol. 1969 Jul;5(1):33–37. doi: 10.1099/0022-1317-5-1-33. [DOI] [PubMed] [Google Scholar]

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