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. 1974 Oct;14(4):924–933. doi: 10.1128/jvi.14.4.924-933.1974

Properties of Intracytoplasmic A Particles Isolated from Oncornavirus-Producing Human Cells

A G Bukrinskaya 1, G G Miller 1, E N Lebedeva 1, L V Agaphonova 1, N N Masurenko 1, K V Ilyin 1
PMCID: PMC355600  PMID: 4138440

Abstract

A particles with the diameter of 70 to 80 nm were isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type. Most of the A particles banded at 1.23 to 1.24 g/ml, whereas 3 to 10% banded at 1.29 g/ml in equilibrium sucrose gradients. They banded at 1.30 g/ml in CsCl gradients suggesting that they contained 8% RNA. Individual A particles sedimented at 200 to 250S in velocity sucrose gradients, but their significant part was found aggregated and sedimented at more than 300S. They were resistant to RNase digestion. A particles possessed polymerase activity which was preferentially activated by Mn2+ rather than by Mg2+, the RNA template being 60S RNA. Cross-hybridization with two DNA products and immunoassay showed that A particles and Mason-Pfizer-like oncornavirus produced by the same cells contained neither homological RNA sequences nor common antigens, suggesting that A particles are not intracellular precursors of Mason-Pfizer-like oncornavirus but represent an independent oncornavirus. Hybridization of A particle RNA with excess of cellular DNA revealed about 20 proviral copies per HEp-2 cell genome and no proviral copies in human embryo and placenta cell genomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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