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. 1974 Oct;14(4):957–964. doi: 10.1128/jvi.14.4.957-964.1974

Structure of Infectious Bursal Disease Virus

K Hirai 1, S Shimakura 1
PMCID: PMC355603  PMID: 4138194

Abstract

Infectious bursal disease virus of chickens was purified, and its structure was examined by the negative-staining technique in the electron microscope. The buoyant density of infectious bursal disease virus in CsCl was found to be 1.34 g/cm3. The morphological details suggest that the capsid of the virion consists of a single layer of 32 capsomeres arranged in 5:3:2 symmetry. The virion measured about 55 nm in diameter and had no envelope.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almeida J. D., Morris R. Antigenically-related viruses associated with infectious bursal disease. J Gen Virol. 1973 Sep;20(3):369–375. doi: 10.1099/0022-1317-20-3-369. [DOI] [PubMed] [Google Scholar]
  2. Amano Y., Katagiri S., Ishida N., Watanabe Y. Spontaneous degradation of reovirus capsid into subunits. J Virol. 1971 Nov;8(5):805–808. doi: 10.1128/jvi.8.5.805-808.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benton W. J., Cover M. S., Rosenberger J. K., Lake R. S. Physicochemical properties of the infectious bursal agent (IBA). Avian Dis. 1967 Aug;11(3):438–445. [PubMed] [Google Scholar]
  4. Bowne J. G., Ritchie A. E. Some morphological features of bluetongue virus. Virology. 1970 Apr;40(4):903–911. doi: 10.1016/0042-6822(70)90136-4. [DOI] [PubMed] [Google Scholar]
  5. CASPAR D. L., KLUG A. Physical principles in the construction of regular viruses. Cold Spring Harb Symp Quant Biol. 1962;27:1–24. doi: 10.1101/sqb.1962.027.001.005. [DOI] [PubMed] [Google Scholar]
  6. Cho Y., Edgar S. A. Characterization of the infectious bursal agent. Poult Sci. 1969 Nov;48(6):2102–2109. doi: 10.3382/ps.0482102. [DOI] [PubMed] [Google Scholar]
  7. Els H. J., Verwoerd D. W. Morphology of bluetongue virus. Virology. 1969 Jun;38(2):213–219. doi: 10.1016/0042-6822(69)90362-6. [DOI] [PubMed] [Google Scholar]
  8. Hirai K., Shimakura S., Chang C., Adachi Y., Kawamoto E. Isolation of infectious bursal disease virus and distribution of precipitating antibodies in chicken sera. Nihon Juigaku Zasshi. 1973 Apr;35(2):105–113. doi: 10.1292/jvms1939.35.105. [DOI] [PubMed] [Google Scholar]
  9. Hosaka Y. A criterion for evaluating the number of capsomeres of icosahedral capsids. Biochim Biophys Acta. 1965 Jun 15;104(1):261–273. doi: 10.1016/0304-4165(65)90243-6. [DOI] [PubMed] [Google Scholar]
  10. Kösters J., Paulsen J. Vermehrung des Erregers der Infektiösen Bursitis der Junghennen (Gumboro-Krankheit) in Hühnerembryonierenzellkultur. Zentralbl Veterinarmed B. 1971 Jul;18(5):366–372. [PubMed] [Google Scholar]
  11. LOH P. C., HOHL H. R., SOERGEL M. FINE STRUCTURE OF REOVIRUS TYPE 2. J Bacteriol. 1965 Apr;89:1140–1144. doi: 10.1128/jb.89.4.1140-1144.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Luftig R. B., Kilham S. S., Hay A. J., Zweerink H. J., Joklik W. K. An ultrastructural study of virions and cores of reovirus type 3. Virology. 1972 Apr;48(1):170–181. doi: 10.1016/0042-6822(72)90124-9. [DOI] [PubMed] [Google Scholar]
  13. Lunger P. D., Maddux T. C. Fine-structure studies of the avian infectious bursal agent. I. In vivo viral morphogenesis. Avian Dis. 1972 Jul-Sep;16(4):874–893. [PubMed] [Google Scholar]
  14. MAYOR H. D., JAMISON R. M., JORDAN L. E., VANMITCHELL M. REOVIRUSES. II. STRUCTURE AND COMPOSITION OF THE VIRION. J Bacteriol. 1965 Jun;89:1548–1556. doi: 10.1128/jb.89.6.1548-1556.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Murphy F. A., Borden E. C., Shope R. E., Harrison A. Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus--a new taxonomic group. Electron microscopic studies. J Gen Virol. 1971 Nov;13(2):273–288. doi: 10.1099/0022-1317-13-2-273. [DOI] [PubMed] [Google Scholar]
  16. STUDDERT M. J. SENSITIVITY OF BLUETONGUE VIRUS TO ETHER AND SODIUM DEOXYCHOLATE. Proc Soc Exp Biol Med. 1965 Apr;118:1006–1009. doi: 10.3181/00379727-118-30030. [DOI] [PubMed] [Google Scholar]
  17. SVEHAG S. E. THERMAL INACTIVATION OF BLUE TONGUE VIRUS. Arch Gesamte Virusforsch. 1963 Aug 26;13:499–510. doi: 10.1007/BF01267793. [DOI] [PubMed] [Google Scholar]
  18. Studdert M. J., Pangborn J., Addison R. B. Bluetongue virus structure. Virology. 1966 Jul;29(3):509–511. doi: 10.1016/0042-6822(66)90232-7. [DOI] [PubMed] [Google Scholar]
  19. Svehag S. E., Leendertsen L., Gorham J. R. Sensitivity of bluetongue virus to lipid solvents, trypsin and pH changes and its serological relationship to arboviruses. J Hyg (Lond) 1966 Sep;64(3):339–346. doi: 10.1017/s0022172400040614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. VASQUEZ C., TOURNIER P. The morphology of reovirus. Virology. 1962 Aug;17:503–510. doi: 10.1016/0042-6822(62)90149-6. [DOI] [PubMed] [Google Scholar]
  21. Verwoerd D. W. Purification and characterization of bluetongue virus. Virology. 1969 Jun;38(2):203–212. doi: 10.1016/0042-6822(69)90361-4. [DOI] [PubMed] [Google Scholar]

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