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. 1974 Oct;14(4):975–980. doi: 10.1128/jvi.14.4.975-980.1974

Diffusion Constant and Dimension of Bacteriophage φX174 as Determined by Self-Beat Laser Light Spectroscopy and Electron Microscopy

M E Bayer 1, R W DeBlois 2
PMCID: PMC355605  PMID: 4138908

Abstract

The diffusion constant of bacteriophage φX174 was determined by laser light self-beat spectroscopy. The method allows one to establish the diffusion constant in comparatively short time and with high accuracy. From the diffusion constant D37 = 1.96 ± 0.08 × 10−7 cm2/s, the size of the virus particles within their aqueous milieu can be calculated. For φX174, a diffusional diameter of 31.4 ± 1.0 nm was found, in good agreement with measurements of diameters of freezeetched (32.3 ± 1.8 nm) and negatively stained particles (33.8 ± 2.1 nm), provided that the entire spikes of the virion are included. Other isometric viruses may show a complex interaction of the virion with the surrounding water and its ions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAYER M. E., ANDERSON T. F. THE PREPARATION OF HOLEY FILMS FOR ELECTRON MICROSCOPY. Experientia. 1963 Aug 15;19:433–434. doi: 10.1007/BF02171534. [DOI] [PubMed] [Google Scholar]
  2. Bayer M. E., Starkey T. W. The adsorption of bacteriophage phi X174 and its interaction with Escherichia coli; a kinetic and morphological study. Virology. 1972 Jul;49(1):236–256. doi: 10.1016/s0042-6822(72)80026-6. [DOI] [PubMed] [Google Scholar]
  3. Brown D. T., MacKenzie J. M., Bayer M. E. Mode of host cell penetration by bacteriophage phi X174. J Virol. 1971 Jun;7(6):836–846. doi: 10.1128/jvi.7.6.836-846.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CASPAR D. L., KLUG A. Physical principles in the construction of regular viruses. Cold Spring Harb Symp Quant Biol. 1962;27:1–24. doi: 10.1101/sqb.1962.027.001.005. [DOI] [PubMed] [Google Scholar]
  5. Harvey J. D. Diffusion coefficients and hydrodynamic radii of three spherical RNA viruses by laser light scattering. Virology. 1973 Nov;56(1):365–368. doi: 10.1016/0042-6822(73)90313-9. [DOI] [PubMed] [Google Scholar]

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