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. Author manuscript; available in PMC: 2014 Jan 18.
Published in final edited form as: Oncogene. 2012 Sep 17;32(29):3461–3469. doi: 10.1038/onc.2012.426

Figure 2. p68 is not required for DNA damage-induced apoptosis or the induction of Bax.

Figure 2

U2OS cells were transfected with non-specific (NS), p68 or p21 siRNAs and treated with doxorubicin prior to analysis by flow cytometry or RNA extraction for qRT-PCR (TaqMan). Untreated cells served as controls. +: cells treated with 1.5μM doxorubicin.

A: Percentage of apoptotic cells as determined by Annexin V staining and flow cytometry.

B-E: Levels of mRNAs as indicated shown relative to respective mRNA cells transfected with a non-specific siRNA and not treated with doxorubicin. In each case this is shown as 1. F: corresponding western blots.

In all graphs the values shown are the averages from 3 independent experiments +/− s.e.m. and statistically significant differences (p=<0.05) are indicated.