Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1974 Nov;14(5):1070–1075. doi: 10.1128/jvi.14.5.1070-1075.1974

Mechanism of Replication of φX174 Single-Stranded DNA IX. Requirement for the Escherichia coli dnaG Protein

Grant McFadden 1, David T Denhardt 1
PMCID: PMC355621  PMID: 4610175

Abstract

Escherichia coli NY73, possessing a temperature-sensitive mutation in the dnaG locus, was rendered sensitive to bacteriophage φX174 by P1 transduction. φX174 reproduces in this strain at 30 C but not at 40 C. All three stages of φX174 replication, parental replicative form (RF) synthesis, RF replication, and progeny single-stranded DNA synthesis, are thermolabile in this mutant. Competition-annealing data show that both plus- and minus-strand synthesis are equally inhibited after shift up to 40 C during RF replication. We conclude that the dnaG gene product is required for the synthesis of both strands of φX RF during RF replication and of the complementary strand and viral progeny strands during stages I and III, respectively.

Full text

PDF
1070

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADELBERG E. A., BURNS S. N. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. doi: 10.1128/jb.79.3.321-330.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  3. Denhardt D. T., Dressler D. H., Hathaway A. THE ABORTIVE REPLICATION OF PhiX174 DNA IN A RECOMBINATION-DEFICIENT MUTANT OF Escherichia coli. Proc Natl Acad Sci U S A. 1967 Mar;57(3):813–820. doi: 10.1073/pnas.57.3.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Denhardt D. T., Iwaya M., McFadden G., Schochetman G. The mechanism of replication of phi X174 single-stranded DNA. VI. Requirements for ribonucleoside triphosphates and DNA polymerase 3. Can J Biochem. 1973 Dec;51(12):1588–1597. doi: 10.1139/o73-214. [DOI] [PubMed] [Google Scholar]
  5. Denhardt D. T., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. 3. Phage maturation and lysis after synchronized infection. J Mol Biol. 1965 Jul;12(3):641–646. doi: 10.1016/s0022-2836(65)80318-7. [DOI] [PubMed] [Google Scholar]
  6. Dressler D. H., Denhardt D. T. Mechanism of replication of phi-X-174 single stranded DNA. Nature. 1968 Jul 27;219(5152):346–351. doi: 10.1038/219346a0. [DOI] [PubMed] [Google Scholar]
  7. Dumas L. B., Miller C. A. Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaE mutant of Escherichia coli C. J Virol. 1973 Jun;11(6):848–855. doi: 10.1128/jvi.11.6.848-855.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eisenberg S., Denhardt D. T. Structure of nascent phiX174 replicative form: evidence for discontinuous DNA replication. Proc Natl Acad Sci U S A. 1974 Mar;71(3):984–988. doi: 10.1073/pnas.71.3.984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fuchs J. A., Karlström H. O., Warner H. R., Reichard P. Defective gene product in dnaF mutant of Escherichia coli. Nat New Biol. 1972 Jul 19;238(81):69–71. doi: 10.1038/newbio238069a0. [DOI] [PubMed] [Google Scholar]
  10. Gefter M. L., Hirota Y., Kornberg T., Wechsler J. A., Barnoux C. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150–3153. doi: 10.1073/pnas.68.12.3150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  12. Greenlee L. L. Replication of bacteriophage phiX-174 in a mutant of Escherichia coli defective in the dnaE gene. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1757–1760. doi: 10.1073/pnas.70.6.1757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gross J. D. DNA replication in bacteria. Curr Top Microbiol Immunol. 1972;57:39–74. doi: 10.1007/978-3-642-65297-4_2. [DOI] [PubMed] [Google Scholar]
  14. Kranias E. G., Dumas L. B. Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaC mutant of escherichia coli C. J Virol. 1974 Jan;13(1):146–154. doi: 10.1128/jvi.13.1.146-154.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  16. Lark K. G. Genetic control over the initiation of the synthesis of the short deoxynucleotide chains in E. coli. Nat New Biol. 1972 Dec 20;240(103):237–240. doi: 10.1038/newbio240237a0. [DOI] [PubMed] [Google Scholar]
  17. Manor H., Deutscher M. P., Littauer U. Z. Rates of DNA chain growth in Escherichia coli. J Mol Biol. 1971 Nov 14;61(3):503–524. doi: 10.1016/0022-2836(71)90062-3. [DOI] [PubMed] [Google Scholar]
  18. SINSHEIMER R. L., STARMAN B., NAGLER C., GUTHRIE S. The process of infection with bacteriophage phi-XI74. I. Evidence for a "replicative form". J Mol Biol. 1962 Mar;4:142–160. doi: 10.1016/s0022-2836(62)80047-3. [DOI] [PubMed] [Google Scholar]
  19. Schekman R. W., Iwaya M., Bromstrup K., Denhardt D. T. The mechanism of replication of phi X174 single-stranded DNA. 3. An enzymic study of the structure of the replicative form II DNA. J Mol Biol. 1971 Apr 28;57(2):177–199. doi: 10.1016/0022-2836(71)90340-8. [DOI] [PubMed] [Google Scholar]
  20. Schekman R., Wickner W., Westergaard O., Brutlag D., Geider K., Bertsch L. L., Kornberg A. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691–2695. doi: 10.1073/pnas.69.9.2691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schröder C. H., Erben E., Kaerner H. C. A rolling circle model of the in vivo replication of bacteriophage phiX174 replicative form DNA: different fate of two types of progeny replicative form. J Mol Biol. 1973 Oct 5;79(4):599–613. doi: 10.1016/0022-2836(73)90066-1. [DOI] [PubMed] [Google Scholar]
  22. Schröder C. H., Kaerner H. C. Replication of bacteriophage phichi174 replicative form DNA in vivo. J Mol Biol. 1972 Nov 14;71(2):351–362. doi: 10.1016/0022-2836(72)90356-7. [DOI] [PubMed] [Google Scholar]
  23. Steinberg R. A., Denhardt D. T. Inhibition of synthesis of phiX174 DNA in a mutant host thermosensitive for DNA synthesis. J Mol Biol. 1968 Nov 14;37(3):525–528. doi: 10.1016/0022-2836(68)90120-4. [DOI] [PubMed] [Google Scholar]
  24. Taketo A. Sensitivity of Escherichia coli to viral nucleic acid. V. Competence of calcium-treated cells. J Biochem. 1972 Oct;72(4):973–979. doi: 10.1093/oxfordjournals.jbchem.a129988. [DOI] [PubMed] [Google Scholar]
  25. Taketo A. Sensitivity of Escherichia coli to viral nucleic acid. VI. Capacity of dna mutants and DNA polymerase-less mutants for multiplication of phiA and phiX 174. Mol Gen Genet. 1973 Mar 27;122(1):15–22. doi: 10.1007/BF00337970. [DOI] [PubMed] [Google Scholar]
  26. Wechsler J. A., Gross J. D. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol Gen Genet. 1971;113(3):273–284. doi: 10.1007/BF00339547. [DOI] [PubMed] [Google Scholar]
  27. Wickner R. B., Wright M., Wickner S., Hurwitz J. Conversion of phiX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3233–3237. doi: 10.1073/pnas.69.11.3233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wickner S., Wright M., Hurwitz J. Association of DNA-dependent and -independent ribonucleoside triphosphatase activities with dnaB gene product of Escherichia coli. Proc Natl Acad Sci U S A. 1974 Mar;71(3):783–787. doi: 10.1073/pnas.71.3.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wickner S., Wright M., Hurwitz J. Studies on in vitro DNA synthesis. Purification of the dna G gene product from Escherichia coli. Proc Natl Acad Sci U S A. 1973 May;70(5):1613–1618. doi: 10.1073/pnas.70.5.1613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wright M., Wickner S., Hurwitz J. Studies on in vitro DNA synthesis. Isolation of DNA B gene product from Escherichia coli. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3120–3124. doi: 10.1073/pnas.70.11.3120. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES