Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1974 Nov;14(5):1169–1178. doi: 10.1128/jvi.14.5.1169-1178.1974

Effect of Interferon Upon the Primary and Secondary Transcription of Vesicular Stomatitis and Influenza Viruses

Patricia Repik 1, Anne Flamand 1, D H L Bishop 1
PMCID: PMC355633  PMID: 4372392

Abstract

The synthesis of viral complementary RNA by vesicular stomatitis virus in permissive cells can be distinguished operationally into two phases, primary transcription, which can be observed in cycloheximide treated cells, and secondary transcription, which represents the further synthesis of viral complementary RNA obtained in untreated cells. Pretreatment of mouse L cells or chicken embryo fibroblasts with the homologous interferon and, for chicken embryo fibroblast cells, poly(rI):poly(rC), inhibits the production of infectious virus and reduces the synthesis of viral complementary RNA to levels comparable to that obtained during primary transcription. Treatment of mouse L cells with interferon plus cycloheximide also gives levels of vesicular stomatitis viral complementary RNA synthesis comparable to that observed with the cycloheximide treatment alone. Similar results have been obtained for interferontreated cells subsequently infected with influenza virus (strain WSN). The results are interpreted as indicating that inhibition of virus development by interferon does not act at the level of primary transcription but rather at an intermediate step between primary and secondary transcription, such as viral protein synthesis.

Full text

PDF
1169

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaslestad H. G., Clark H. F., Bishop D. H., Koprowski H. Comparison of the ribonucleic acid polymerases of two rhabdoviruses, Kern Canyon virus and vesicular stomatitis virus. J Virol. 1971 Jun;7(6):726–735. doi: 10.1128/jvi.7.6.726-735.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Asculai S. S., Kuchler R. J. The relationship between cytotoxicity and interferon induction in L-M strain mouse fibroblasts. Proc Soc Exp Biol Med. 1973 May;143(1):252–255. doi: 10.3181/00379727-143-37297. [DOI] [PubMed] [Google Scholar]
  3. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bean W. J., Jr, Simpson R. W. Primary transcription of the influenza virus genome in permissive cells. Virology. 1973 Dec;56(2):646–651. doi: 10.1016/0042-6822(73)90067-6. [DOI] [PubMed] [Google Scholar]
  5. Bialy H. S., Colby C. Inhibition of early vaccinia virus ribonucleic acid synthesis in interferon-treated chicken embryo fibroblasts. J Virol. 1972 Feb;9(2):286–289. doi: 10.1128/jvi.9.2.286-289.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bishop D. H., Obijeski J. F., Simpson R. W. Transcription of the influenza ribonucleic acid genome by a virion polymerase. I. Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase. J Virol. 1971 Jul;8(1):66–73. doi: 10.1128/jvi.8.1.66-73.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chow N. L., Simpson R. W. RNA-dependent RNA polymerase activity associated with virions and subviral particles of myxoviruses. Proc Natl Acad Sci U S A. 1971 Apr;68(4):752–756. doi: 10.1073/pnas.68.4.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Colby C., Morgan M. J. Interferon induction and action. Annu Rev Microbiol. 1971;25:333–360. doi: 10.1146/annurev.mi.25.100171.002001. [DOI] [PubMed] [Google Scholar]
  9. Falcoff E., Falcoff R., Lebleu B., Revel M. Correlation between the antiviral effect of interferon treatment and the inhibition of in vitro mRNA translation in noninfected L cells. J Virol. 1973 Sep;12(3):421–430. doi: 10.1128/jvi.12.3.421-430.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Field A. K., Tytell A. A., Lampson G. P., Hilleman M. R. Inducers of interferon and host resistance, V. In vitro studies. Proc Natl Acad Sci U S A. 1968 Sep;61(1):340–346. doi: 10.1073/pnas.61.1.340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Flamand A., Bishop D. H. Primary in vivo transcription of vesicular stomatitis virus and temperature-sensitive mutants of five vesicular stomatitis virus complementation groups. J Virol. 1973 Dec;12(6):1238–1252. doi: 10.1128/jvi.12.6.1238-1252.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gupta S. L., Sopori M. L., Lengyel P. Inhibition of protein synthesis directed by added viral and cellular messenger RNAs in extracts of interferon-treated Ehrlich ascites tumor cells. Location and dominance of the inhibitor(s). Biochem Biophys Res Commun. 1973 Sep 18;54(2):777–783. doi: 10.1016/0006-291x(73)91491-5. [DOI] [PubMed] [Google Scholar]
  13. Jordan G. W., Merigan T. C. Quantitative aspects of interferon-induced plaque reduction: effects of cell age and dose of challenge virus. Proc Soc Exp Biol Med. 1974 Mar;145(3):1037–1041. doi: 10.3181/00379727-145-37948. [DOI] [PubMed] [Google Scholar]
  14. Manders E. K., Tilles J. G., Huang A. S. Interferon-mediated inhibition of virion-directed transcription. Virology. 1972 Aug;49(2):573–581. doi: 10.1016/0042-6822(72)90508-9. [DOI] [PubMed] [Google Scholar]
  15. Marcus P. I., Engelhardt D. L., Hunt J. M., Sekellick M. J. Interferon action: inhibition of vesicular stomatitis virus RNA synthesis induced by virion-bound polymerase. Science. 1971 Nov 5;174(4009):593–598. doi: 10.1126/science.174.4009.593. [DOI] [PubMed] [Google Scholar]
  16. Metz D. H., Esteban M. Interferon inhibits viral protein synthesis in L cells infected with vaccinia virus. Nature. 1972 Aug 18;238(5364):385–388. doi: 10.1038/238385a0. [DOI] [PubMed] [Google Scholar]
  17. SIMPSON R. W., HIRST G. K. Genetic recombination among influenza viruses. I. Cross reactivation of plaque-forming capacity as a method for selecting recombinants from the progeny of crosses between influenza A strains. Virology. 1961 Dec;15:436–451. doi: 10.1016/0042-6822(61)90111-8. [DOI] [PubMed] [Google Scholar]
  18. Samuel C. E., Joklik W. K. A protein synthesizing system from interferon-treated cells that discriminates between cellular and viral messenger RNAs. Virology. 1974 Apr;58(2):476–491. doi: 10.1016/0042-6822(74)90082-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES