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. 1974 Dec;14(6):1380–1387. doi: 10.1128/jvi.14.6.1380-1387.1974

Studies on an Endonuclease Activity Associated with the Bacteriophage T7 DNA-Membrane Complex

Rodulfo P Pacumbaba 1, Melvin S Center 1
PMCID: PMC355665  PMID: 4610184

Abstract

Infection of Escherichia coli with bacteriophage T7 results in the formation of an endonuclease which is selectively associated with the T7 DNA-membrane complex. A specificity of association with the complex is indicated by the finding that the enzyme is completely resolved from a previously described T7 endonuclease I. When membrane complexes containing 3H-labeled in vivo synthesized DNA are incubated in the standard reaction mixture a specific cleavage product is formed which is about one-fourth the size of T7 DNA. The endonuclease associated with the complex produces a similar cleavage product after extensive incubation with native T7 DNA or T7 concatemers. Degradation of concatemers occurs by a mechanism in which the DNA is converted to molecules one-half the size of T7. This product is in turn converted to fragments one-fourth the size of mature phage DNA. The endonuclease is not present in membrane complexes from uninfected cells or cells infected with gene 1 mutants. The enzyme activity is, however, present in cells infected with mutants defective in T7 DNA synthesis or maturation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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