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. 1974 Dec;14(6):1627–1629. doi: 10.1128/jvi.14.6.1627-1629.1974

In Vitro Radioisotopic Labeling of Proteins Associated with Purified Polyoma Virions

Janis McMillen 1, R A Consigli 1
PMCID: PMC355699  PMID: 4372416

Abstract

An in vitro procedure using [14C]formaldehyde and sodium borohydride was used to label the proteins of purified polyoma virions. This method, which labeled the three capsid proteins and the four internal histones of polyoma virus, did not alter the biological and biophysical characteristics of the virus. In addition to the seven viral proteins, five additional radioactive peaks were resolved on polyacrylamide gels. Four of these peaks could be attributed to growing the virus in the presence of serum. The fifth peak appears to be a nonhistone host-contributed protein made before infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Consigli R. A., Minocha H. C., Abo-Ahmed H. Multiplication of polyoma virus. II. Source of constituents for viral deoxyribonucleic acid and protein synthesis. J Bacteriol. 1966 Sep;92(3):789–791. doi: 10.1128/jb.92.3.789-791.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Crawford L. V., Gesteland R. F. Synthesis of polyoma proteins in vitro. J Mol Biol. 1973 Mar 15;74(4):627–634. doi: 10.1016/0022-2836(73)90053-3. [DOI] [PubMed] [Google Scholar]
  3. Fritz R. B. Enzymatic radioiodination of the envelope proteins of avian myeloblastosis virus. J Virol. 1974 Jan;13(1):42–45. doi: 10.1128/jvi.13.1.42-45.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hubbard A. L., Cohn Z. A. The enzymatic iodination of the red cell membrane. J Cell Biol. 1972 Nov;55(2):390–405. doi: 10.1083/jcb.55.2.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Khare G. P., Consigli R. A. Cytologic studies in polyoma virus-infected mouse embryo cells. Am J Vet Res. 1967 Sep;28(126):1527–1536. [PubMed] [Google Scholar]
  6. Marchalonis J. J. An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem J. 1969 Jun;113(2):299–305. doi: 10.1042/bj1130299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McMillen J., Consigli R. A. Characterization of polyoma DNA-protein complexes. I. Electrophoretic identification of the proteins in a nucleoprotein complex isolated from polyoma-infected cells. J Virol. 1974 Dec;14(6):1326–1336. doi: 10.1128/jvi.14.6.1326-1336.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Oliphant G., Brackett B. G. Preparation of C 14 labelled antibodies. Immunochemistry. 1972 Dec;9(12):1259–1261. doi: 10.1016/0019-2791(72)90303-5. [DOI] [PubMed] [Google Scholar]
  9. Phillips D. R., Morrison M. The arrangement of proteins in the human erythrocyte membrane. Biochem Biophys Res Commun. 1970 Jul 27;40(2):284–289. doi: 10.1016/0006-291x(70)91007-7. [DOI] [PubMed] [Google Scholar]
  10. Rifkin D. B., Compans R. W., Reich E. A specific labeling procedure for proteins on the outer surface of membranes. J Biol Chem. 1972 Oct 25;247(20):6432–6437. [PubMed] [Google Scholar]
  11. Sefton B. M., Wickus G. G., Burge B. W. Enzymatic iodination of Sindbis virus proteins. J Virol. 1973 May;11(5):730–735. doi: 10.1128/jvi.11.5.730-735.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith G. L., Consigli R. A. Transient inhibition of polyoma virus synthesis by Sendai virus (parainfluenza I). I. Demonstration and nature of the inhibition by inactivated virus. J Virol. 1972 Dec;10(6):1091–1097. doi: 10.1128/jvi.10.6.1091-1097.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stanley P., Haslam E. A. The polypeptides of influenza virus. V. Localization of polypeptides in the virion by iodination techniques. Virology. 1971 Dec;46(3):764–773. doi: 10.1016/0042-6822(71)90078-x. [DOI] [PubMed] [Google Scholar]
  14. Stark G. R., Crawford L. V. Specific labelling of virus proteins by quantitative reaction of cysteine residues with 35 S-sulphite. Nat New Biol. 1972 May 31;237(74):146–147. doi: 10.1038/newbio237146a0. [DOI] [PubMed] [Google Scholar]
  15. Uhr J. W., Vitetta E. S. Synthesis, biochemistry and dynamics of cell surface immunoglobulin on lymphocytes. Fed Proc. 1973 Jan;32(1):35–40. [PubMed] [Google Scholar]
  16. Walter G., Mudd J. A. Iodination of vesicular stomatitis virus with lactoperoxidase. Virology. 1973 Apr;52(2):574–577. doi: 10.1016/0042-6822(73)90353-x. [DOI] [PubMed] [Google Scholar]
  17. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  18. Witte O. N., Weissman I. L., Kaplan H. S. Structural characteristics of some murine RNA tumor viruses studied by lactoperoxidase iodination. Proc Natl Acad Sci U S A. 1973 Jan;70(1):36–40. doi: 10.1073/pnas.70.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]

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