Abstract
Nonpermissive cells infected with φX174 gene D amber mutants synthesized some sixfold less viral RNA than permissive cells. The decrease was unaffected by increasing the multiplicity of infection and was a consequence of an overall decrease in all viral RNA species. It is suggested that the gene D product may function in replicative form DNA unwinding to expose the template for transcription.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alberts B., Frey L., Delius H. Isolation and characterization of gene 5 protein of filamentous bacterial viruses. J Mol Biol. 1972 Jul 14;68(1):139–152. doi: 10.1016/0022-2836(72)90269-0. [DOI] [PubMed] [Google Scholar]
- Benbow R. M., Mayol R. F., Picchi J. C., Sinsheimer R. L. Direction of Translation and Size of Bacteriophage phiX174 Cistrons. J Virol. 1972 Jul;10(1):99–114. doi: 10.1128/jvi.10.1.99-114.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Godson G. N. Characterization and synthesis of phi X174 proteins in ultraviolet-irradiated and unirradiated cells. J Mol Biol. 1971 May 14;57(3):541–553. doi: 10.1016/0022-2836(71)90108-2. [DOI] [PubMed] [Google Scholar]
- HAYASHI M., HAYASHI M. N., SPIEGELMAN S. RESTRICTION OF IN VIVO GENETIC TRANSCRIPTION TO ONE OF THE COMPLEMENTARY STRANDS OF DNA. Proc Natl Acad Sci U S A. 1963 Oct;50:664–672. doi: 10.1073/pnas.50.4.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutchison C. A., 3rd, Sinsheimer R. L. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene. J Mol Biol. 1966 Jul;18(3):429–447. doi: 10.1016/s0022-2836(66)80035-9. [DOI] [PubMed] [Google Scholar]
- Iwaya M., Denhardt D. T. The mechanism of replication of phi X174 single-stranded DNA. II. The role of viral proteins. J Mol Biol. 1971 Apr 28;57(2):159–175. doi: 10.1016/0022-2836(71)90339-1. [DOI] [PubMed] [Google Scholar]
- Lindqvist B. H., Sinsheimer R. L. Process of infection with bacteriophage phi-X174. XIV. Studies on macromolecular synthesis during infection with a lysis-defective mutant. J Mol Biol. 1967 Aug 28;28(1):87–94. doi: 10.1016/s0022-2836(67)80079-2. [DOI] [PubMed] [Google Scholar]
- Lindqvist B. H., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. XV. Bacteriophage DNA synthesis in abortive infections with a set of conditional lethal mutants. J Mol Biol. 1967 Nov 28;30(1):69–80. doi: 10.1016/0022-2836(67)90244-6. [DOI] [PubMed] [Google Scholar]
- Oey J. L., Knippers R. Properties of the isolated gene 5 protein of bacteriophage fd. J Mol Biol. 1972 Jul 14;68(1):125–138. doi: 10.1016/0022-2836(72)90268-9. [DOI] [PubMed] [Google Scholar]
- Pratt D., Laws P., Griffith J. Complex of bacteriophage M13 single-stranded DNA and gene 5 protein. J Mol Biol. 1974 Feb 5;82(4):425–439. doi: 10.1016/0022-2836(74)90239-3. [DOI] [PubMed] [Google Scholar]
- Puga A., Tessman I. Mechanism of transcription of bacteriophage S13. I. Dependence of messengerRNA synthesis on amount and configuration of DNA. J Mol Biol. 1973 Mar 25;75(1):83–97. doi: 10.1016/0022-2836(73)90530-5. [DOI] [PubMed] [Google Scholar]
- SINSHEIMER R. L., STARMAN B., NAGLER C., GUTHRIE S. The process of infection with bacteriophage phi-XI74. I. Evidence for a "replicative form". J Mol Biol. 1962 Mar;4:142–160. doi: 10.1016/s0022-2836(62)80047-3. [DOI] [PubMed] [Google Scholar]
- Sedat J., Lyon A., Sinsheimer R. L. Purification of Escherichia coli pulse-labeled RNA by benzoylated DEAE-cellulose chromatography. J Mol Biol. 1969 Sep 28;44(3):415–434. doi: 10.1016/0022-2836(69)90370-2. [DOI] [PubMed] [Google Scholar]
- Tessman E. S. Mutants of bacteriophage S13 blocked in infectious DNA synthesis. J Mol Biol. 1966 May;17(1):218–236. doi: 10.1016/s0022-2836(66)80104-3. [DOI] [PubMed] [Google Scholar]