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. 1975 Nov;16(5):1095–1100. doi: 10.1128/jvi.16.5.1095-1100.1975

DNA polymerases in polyoma virus-infected mouse kidney cells.

U Wintersberger, E Wintersberger
PMCID: PMC355706  PMID: 171441

Abstract

Infection of arrested mouse kidney cells by polyoma virus results in the induction of the cellular 6-8S DNA polymerase activity. Levels of this enzyme increase two- to threefold in the cytoplasm but seven- to tenfold in nuclei and nuclear extract, suggesting an accumulation of the enzyme in the nucleus. Experiments using the inhibitor of DNA synthesis, fluordeoxyuridine, indicate that this accumulation is linked to active DNA synthesis. The activity and cellular distribution of the small 3.4S DNA polymerase remains unchanged.

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Selected References

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  1. Baril E. F., Brown O. E., Jenkins M. D., Laszlo J. Deoxyribonucleic acid polymerase with rat liver ribosomes and smooth membranes. Purification and properties of the enzymes. Biochemistry. 1971 May 25;10(11):1981–1992. doi: 10.1021/bi00787a004. [DOI] [PubMed] [Google Scholar]
  2. Baril E. F., Jenkins M. D., Brown O. E., Laszlo J., Morris H. P. DNA polymerases I and II in regenerating rat liver and Morris hepatomas. Cancer Res. 1973 Jun;33(6):1187–1193. [PubMed] [Google Scholar]
  3. Chang L. M., Bollum F. J. Low molecular weight deoxyribonucleic acid polymerase from rabbit bone marrow. Biochemistry. 1972 Mar 28;11(7):1264–1272. doi: 10.1021/bi00757a023. [DOI] [PubMed] [Google Scholar]
  4. Chang L. M., Bollum F. J. Low molecular weight deoxyribonucleic acid polymerase in mammalian cells. J Biol Chem. 1971 Sep 25;246(18):5835–5837. [PubMed] [Google Scholar]
  5. Chang L. M., Brown M., Bollum F. J. Induction of DNA polymerase in mouse L cells. J Mol Biol. 1973 Feb 15;74(1):1–8. doi: 10.1016/0022-2836(73)90349-5. [DOI] [PubMed] [Google Scholar]
  6. Crawford L. V., Syrett C., Wilde A. The replication of polyoma DNA. J Gen Virol. 1973 Dec;21(3):515–521. doi: 10.1099/0022-1317-21-3-515. [DOI] [PubMed] [Google Scholar]
  7. DULBECCO R., HARTWELL L. H., VOGT M. INDUCTION OF CELLULAR DNA SYNTHESIS BY POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Feb;53:403–410. doi: 10.1073/pnas.53.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fansler B., Loeb L. A. Sea urchin nuclear DNA polymerase. IV. Reversible association of DNA polymerase with nuclei during the cell cycle. Exp Cell Res. 1972 Dec;75(2):433–441. doi: 10.1016/0014-4827(72)90450-8. [DOI] [PubMed] [Google Scholar]
  9. Francke B., Hunter T. In vitro polyoma DNA synthesis: discontinuous chain growth. J Mol Biol. 1974 Feb 15;83(1):99–121. doi: 10.1016/0022-2836(74)90426-4. [DOI] [PubMed] [Google Scholar]
  10. Hartwell L. H., Vogt M., Dulbecco R. Induction of cellular DNA synthesis by polyoma virus. II. Increase in the rate of enzyme synthesis after infection with polyoma virus in mouse kidney cells. Virology. 1965 Nov;27(3):262–272. doi: 10.1016/0042-6822(65)90105-4. [DOI] [PubMed] [Google Scholar]
  11. Hunter T., Francke B. In vitro polyoma DNA synthesis: characterization of a system from infected 3T3 cells. J Virol. 1974 Jan;13(1):125–139. doi: 10.1128/jvi.13.1.125-139.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hunter T., Francke B. Letter: In vitro polyoma DNA synthesis: involvement of RNA in discontinuous chain growth. J Mol Biol. 1974 Feb 15;83(1):123–130. doi: 10.1016/0022-2836(74)90427-6. [DOI] [PubMed] [Google Scholar]
  13. Kit S., Dubbs D. R., Frearson P. M. Enzymes of nucleic acid metabolism in cells infected with polyoma virus. Cancer Res. 1966 Apr;26(4):638–646. [PubMed] [Google Scholar]
  14. Kára J., Weil R. Specific activation of the DNA-synthesizing apparatus in contact-inhibited mouse kidney cells by polyoma virus. Proc Natl Acad Sci U S A. 1967 Jan;57(1):63–70. doi: 10.1073/pnas.57.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Magnusson G., Pigiet V., Winnacker E. L., Abrams R., Reichard P. RNA-linked short DNA fragments during polyoma replication. Proc Natl Acad Sci U S A. 1973 Feb;70(2):412–415. doi: 10.1073/pnas.70.2.412. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Magnusson G., Winnacker E. L., Eliasson R., Reichard P. Replication of polyoma DNA in isolated nuclei. II. Evidence for semi-conservative replication. J Mol Biol. 1972 Dec 30;72(3):539–552. doi: 10.1016/0022-2836(72)90173-8. [DOI] [PubMed] [Google Scholar]
  18. Pigiet V., Eliasson R., Reichard P. Replication of polyoma DNA in isolated nuclei. 3. The nucleotide sequence at the RNA-DNA junction of nascent strands. J Mol Biol. 1974 Mar 25;84(1):197–216. doi: 10.1016/0022-2836(74)90222-8. [DOI] [PubMed] [Google Scholar]
  19. Pétursson G., Weil R. A study on the mechanism of polyoma-induced activation of the cellular DNA-synthesizing apparatus. Synchronization by FUdR of virus-induced DNA synthesis. Arch Gesamte Virusforsch. 1968;24(1):1–29. doi: 10.1007/BF01242898. [DOI] [PubMed] [Google Scholar]
  20. Sedwick W. D., Wang T. S., Korn D. Purification and properties of nuclear and cytoplasmic deoxyribonucleic acid polymerases from human KB cells. J Biol Chem. 1972 Aug 25;247(16):5026–5033. [PubMed] [Google Scholar]
  21. Spadari S., Weissbach A. RNA-primed DNA synthesis: specific catalysis by HeLa cell DNA polymerase alpha. Proc Natl Acad Sci U S A. 1975 Feb;72(2):503–507. doi: 10.1073/pnas.72.2.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Spadari S., Weissbach A. The interrelation between DNA synthesis and various DNA polymerase activities in synchronized HeLa cells. J Mol Biol. 1974 Jun 15;86(1):11–20. doi: 10.1016/s0022-2836(74)80003-3. [DOI] [PubMed] [Google Scholar]
  23. WINOCOUR E. Purification of polyoma virus. Virology. 1963 Feb;19:158–168. doi: 10.1016/0042-6822(63)90005-9. [DOI] [PubMed] [Google Scholar]
  24. Weil R., Kára J. Polyoma "tumor antigen": an activator of chromosome replication? Proc Natl Acad Sci U S A. 1970 Oct;67(2):1011–1017. doi: 10.1073/pnas.67.2.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weil R., Michel M. R., Ruschmann G. K. Induction of cellular DNA synthesis by polyoma virus. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1468–1475. doi: 10.1073/pnas.53.6.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weissbach A., Schlabach A., Fridlender B., Bolden A. DNA polymerases from human cells. Nat New Biol. 1971 Jun 9;231(23):167–170. doi: 10.1038/newbio231167a0. [DOI] [PubMed] [Google Scholar]
  27. Winnacker E. L., Magnusson G., Reichard P. Replication of polyoma DNA in isolated nuclei. I. Characterization of the system from mouse fibroblast 3T6 cells. J Mol Biol. 1972 Dec 30;72(3):523–537. doi: 10.1016/0022-2836(72)90172-6. [DOI] [PubMed] [Google Scholar]
  28. Wintersberger U., Wintersberger E. Studies on deoxyribonucleic acid polymerases from yeast. 1. Parial purification and properties of two DNA polymerases from mitochondria-free cell extracts. Eur J Biochem. 1970 Mar 1;13(1):11–19. doi: 10.1111/j.1432-1033.1970.tb00893.x. [DOI] [PubMed] [Google Scholar]

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